Molecular Occupancy of Nanodot Arrays
Single-molecule nanodot arrays, in which a biomolecule of choice (protein, nucleic acid, etc.) is bound to a metallic nanoparticle on a solid substrate, are becoming an increasingly important tool in the study of biomolecular and cellular interactions. We have developed an on-chip measurement protoc...
| मुख्य लेखकों: | , , , , , |
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| स्वरूप: | Journal article |
| भाषा: | English |
| प्रकाशित: |
American Chemical Society
2016
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| _version_ | 1826271906696790016 |
|---|---|
| author | Cai, H Wolfenson, H Depoil, D Dustin, M Sheetz, M Wind, S |
| author_facet | Cai, H Wolfenson, H Depoil, D Dustin, M Sheetz, M Wind, S |
| author_sort | Cai, H |
| collection | OXFORD |
| description | Single-molecule nanodot arrays, in which a biomolecule of choice (protein, nucleic acid, etc.) is bound to a metallic nanoparticle on a solid substrate, are becoming an increasingly important tool in the study of biomolecular and cellular interactions. We have developed an on-chip measurement protocol to monitor and control the molecular occupancy of nanodots. Arrays of widely spaced nanodots and nanodot clusters were fabricated on glass surfaces by nanolithography and functionalized with fluorescently labeled proteins. The molecular occupancy was determined by monitoring individual fluorophore bleaching events, while accounting for fluorescence quenching effects. We found that the occupancy can be interpreted as a packing problem, and depends on nanodot size and binding ligand concentration, where the latter is easily adjusted to compensate the flexibility of dimension control in nanofabrication. The results are scalable with nanodot cluster size, extending to large area close packed arrays. As an example, the nanoarray platform was used to probe the geometric requirement of T-cell activation at the single-molecule level. |
| first_indexed | 2024-03-06T22:04:07Z |
| format | Journal article |
| id | oxford-uuid:4f94b7f4-8e50-4bfa-846a-1acae4a1bd4c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:04:07Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:4f94b7f4-8e50-4bfa-846a-1acae4a1bd4c2022-03-26T16:08:03ZMolecular Occupancy of Nanodot ArraysJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4f94b7f4-8e50-4bfa-846a-1acae4a1bd4cEnglishSymplectic Elements at OxfordAmerican Chemical Society2016Cai, HWolfenson, HDepoil, DDustin, MSheetz, MWind, SSingle-molecule nanodot arrays, in which a biomolecule of choice (protein, nucleic acid, etc.) is bound to a metallic nanoparticle on a solid substrate, are becoming an increasingly important tool in the study of biomolecular and cellular interactions. We have developed an on-chip measurement protocol to monitor and control the molecular occupancy of nanodots. Arrays of widely spaced nanodots and nanodot clusters were fabricated on glass surfaces by nanolithography and functionalized with fluorescently labeled proteins. The molecular occupancy was determined by monitoring individual fluorophore bleaching events, while accounting for fluorescence quenching effects. We found that the occupancy can be interpreted as a packing problem, and depends on nanodot size and binding ligand concentration, where the latter is easily adjusted to compensate the flexibility of dimension control in nanofabrication. The results are scalable with nanodot cluster size, extending to large area close packed arrays. As an example, the nanoarray platform was used to probe the geometric requirement of T-cell activation at the single-molecule level. |
| spellingShingle | Cai, H Wolfenson, H Depoil, D Dustin, M Sheetz, M Wind, S Molecular Occupancy of Nanodot Arrays |
| title | Molecular Occupancy of Nanodot Arrays |
| title_full | Molecular Occupancy of Nanodot Arrays |
| title_fullStr | Molecular Occupancy of Nanodot Arrays |
| title_full_unstemmed | Molecular Occupancy of Nanodot Arrays |
| title_short | Molecular Occupancy of Nanodot Arrays |
| title_sort | molecular occupancy of nanodot arrays |
| work_keys_str_mv | AT caih molecularoccupancyofnanodotarrays AT wolfensonh molecularoccupancyofnanodotarrays AT depoild molecularoccupancyofnanodotarrays AT dustinm molecularoccupancyofnanodotarrays AT sheetzm molecularoccupancyofnanodotarrays AT winds molecularoccupancyofnanodotarrays |