Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement.
Graft-versus-host disease is one of the major problems in clinical bone marrow transplantation. Many experiments in animals have shown that it could be greatly reduced if mature T lymphocytes were removed from the donor marrow. Here we describe a new rat monoclonal antibody, CAMPATH 1, which is suit...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1983
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| _version_ | 1797068020466581504 |
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| author | Hale, G Bright, S Chumbley, G Hoang, T Metcalf, D Munro, A Waldmann, H |
| author_facet | Hale, G Bright, S Chumbley, G Hoang, T Metcalf, D Munro, A Waldmann, H |
| author_sort | Hale, G |
| collection | OXFORD |
| description | Graft-versus-host disease is one of the major problems in clinical bone marrow transplantation. Many experiments in animals have shown that it could be greatly reduced if mature T lymphocytes were removed from the donor marrow. Here we describe a new rat monoclonal antibody, CAMPATH 1, which is suitable for depleting lymphocytes from human marrow grafts. CAMPATH 1 is an IgM that fixes human complement. It binds to both T and B lymphocytes and some monocytes but not to other hemopoietic cells. When peripheral blood mononuclear cells were treated with CAMPATH 1 and complement, more than 99% of lymphocytes were killed and viable T cells could no longer be detected. Under these conditions, in vitro multipotential erythroid and myeloid colony-forming cells were unaffected. As well as being used for in vitro treatment of bone marrow to remove T cells, CAMPATH 1 could potentially be applied to other experimental and clinical situations where depletion of lymphoid cells is required, including serotherapy to achieve immunosuppression for organ transplants or to treat lymphocytic leukemias. |
| first_indexed | 2024-03-06T22:04:42Z |
| format | Journal article |
| id | oxford-uuid:4fbf459b-063c-43d7-adad-1f945ad5f070 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:04:42Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:4fbf459b-063c-43d7-adad-1f945ad5f0702022-03-26T16:09:23ZRemoval of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4fbf459b-063c-43d7-adad-1f945ad5f070EnglishSymplectic Elements at Oxford1983Hale, GBright, SChumbley, GHoang, TMetcalf, DMunro, AWaldmann, HGraft-versus-host disease is one of the major problems in clinical bone marrow transplantation. Many experiments in animals have shown that it could be greatly reduced if mature T lymphocytes were removed from the donor marrow. Here we describe a new rat monoclonal antibody, CAMPATH 1, which is suitable for depleting lymphocytes from human marrow grafts. CAMPATH 1 is an IgM that fixes human complement. It binds to both T and B lymphocytes and some monocytes but not to other hemopoietic cells. When peripheral blood mononuclear cells were treated with CAMPATH 1 and complement, more than 99% of lymphocytes were killed and viable T cells could no longer be detected. Under these conditions, in vitro multipotential erythroid and myeloid colony-forming cells were unaffected. As well as being used for in vitro treatment of bone marrow to remove T cells, CAMPATH 1 could potentially be applied to other experimental and clinical situations where depletion of lymphoid cells is required, including serotherapy to achieve immunosuppression for organ transplants or to treat lymphocytic leukemias. |
| spellingShingle | Hale, G Bright, S Chumbley, G Hoang, T Metcalf, D Munro, A Waldmann, H Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement. |
| title | Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement. |
| title_full | Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement. |
| title_fullStr | Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement. |
| title_full_unstemmed | Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement. |
| title_short | Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement. |
| title_sort | removal of t cells from bone marrow for transplantation a monoclonal antilymphocyte antibody that fixes human complement |
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