Peptidomimetic bond formation by DNA-templated acyl transfer.
The efficiencies of DNA-templated acyl transfer reactions between a thioester modified oligonucleotide and a series of amine and thiol based nucleophiles are directly compared. The reactivity of the nucleophile, reaction conditions (solvent, buffer, pH) and linker length all play important roles in...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1826271950433943552 |
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| author | Mckee, M Evans, A Gerrard, SR O'Reilly, R Turberfield, A Stulz, E |
| author_facet | Mckee, M Evans, A Gerrard, SR O'Reilly, R Turberfield, A Stulz, E |
| author_sort | Mckee, M |
| collection | OXFORD |
| description | The efficiencies of DNA-templated acyl transfer reactions between a thioester modified oligonucleotide and a series of amine and thiol based nucleophiles are directly compared. The reactivity of the nucleophile, reaction conditions (solvent, buffer, pH) and linker length all play important roles in determining the efficiency of the transfer reaction. Careful optimisation of the system enables the use of DNA-templated synthesis to form stable peptide-like bonds under mild aqueous conditions close to neutral pH. |
| first_indexed | 2024-03-06T22:04:49Z |
| format | Journal article |
| id | oxford-uuid:4fc8c7d9-4bc9-4525-aa83-cfdfdef2f347 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:04:49Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:4fc8c7d9-4bc9-4525-aa83-cfdfdef2f3472022-03-26T16:09:34ZPeptidomimetic bond formation by DNA-templated acyl transfer.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4fc8c7d9-4bc9-4525-aa83-cfdfdef2f347EnglishSymplectic Elements at Oxford2011Mckee, MEvans, AGerrard, SRO'Reilly, RTurberfield, AStulz, EThe efficiencies of DNA-templated acyl transfer reactions between a thioester modified oligonucleotide and a series of amine and thiol based nucleophiles are directly compared. The reactivity of the nucleophile, reaction conditions (solvent, buffer, pH) and linker length all play important roles in determining the efficiency of the transfer reaction. Careful optimisation of the system enables the use of DNA-templated synthesis to form stable peptide-like bonds under mild aqueous conditions close to neutral pH. |
| spellingShingle | Mckee, M Evans, A Gerrard, SR O'Reilly, R Turberfield, A Stulz, E Peptidomimetic bond formation by DNA-templated acyl transfer. |
| title | Peptidomimetic bond formation by DNA-templated acyl transfer. |
| title_full | Peptidomimetic bond formation by DNA-templated acyl transfer. |
| title_fullStr | Peptidomimetic bond formation by DNA-templated acyl transfer. |
| title_full_unstemmed | Peptidomimetic bond formation by DNA-templated acyl transfer. |
| title_short | Peptidomimetic bond formation by DNA-templated acyl transfer. |
| title_sort | peptidomimetic bond formation by dna templated acyl transfer |
| work_keys_str_mv | AT mckeem peptidomimeticbondformationbydnatemplatedacyltransfer AT evansa peptidomimeticbondformationbydnatemplatedacyltransfer AT gerrardsr peptidomimeticbondformationbydnatemplatedacyltransfer AT oreillyr peptidomimeticbondformationbydnatemplatedacyltransfer AT turberfielda peptidomimeticbondformationbydnatemplatedacyltransfer AT stulze peptidomimeticbondformationbydnatemplatedacyltransfer |