Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling
The quantitative analysis of metabolic networks is a prerequisite for understanding the integration and regulation of plant metabolism and for devising rational approaches for manipulating resource allocation in plants. The analysis of steady state stable isotope labelling experiments using nuclear...
| Principais autores: | , , |
|---|---|
| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2003
|
| _version_ | 1826271969637564416 |
|---|---|
| author | Kruger, N Ratcliffe, R Roscher, A |
| author_facet | Kruger, N Ratcliffe, R Roscher, A |
| author_sort | Kruger, N |
| collection | OXFORD |
| description | The quantitative analysis of metabolic networks is a prerequisite for understanding the integration and regulation of plant metabolism and for devising rational approaches for manipulating resource allocation in plants. The analysis of steady state stable isotope labelling experiments using nuclear magnetic resonance (NMR) spectroscopy has developed into a powerful method for determining these fluxes in micro-organisms and its application to heterotrophic plant metabolism is increasing. After an introductory discussion of the well known role of stable isotopes in pathway delineation, the review considers their application to metabolic flux analysis in plants. These applications are divided into two groups - small scale analyses of fluxes through particular pathways and large scale analyses of multiple fluxes through metabolic networks - and the problems caused by the complexity of intermediary metabolism in plants are discussed. It is concluded that metabolic flux analysis provides a powerful method for defining the metabolic phenotype of wild type, mutant and transgenic plants and that its development should be pursued. |
| first_indexed | 2024-03-06T22:05:08Z |
| format | Journal article |
| id | oxford-uuid:4fe0b52f-b5f6-41be-863e-c84b7fbd75e0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:05:08Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:4fe0b52f-b5f6-41be-863e-c84b7fbd75e02022-03-26T16:10:15ZQuantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labellingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4fe0b52f-b5f6-41be-863e-c84b7fbd75e0EnglishSymplectic Elements at Oxford2003Kruger, NRatcliffe, RRoscher, AThe quantitative analysis of metabolic networks is a prerequisite for understanding the integration and regulation of plant metabolism and for devising rational approaches for manipulating resource allocation in plants. The analysis of steady state stable isotope labelling experiments using nuclear magnetic resonance (NMR) spectroscopy has developed into a powerful method for determining these fluxes in micro-organisms and its application to heterotrophic plant metabolism is increasing. After an introductory discussion of the well known role of stable isotopes in pathway delineation, the review considers their application to metabolic flux analysis in plants. These applications are divided into two groups - small scale analyses of fluxes through particular pathways and large scale analyses of multiple fluxes through metabolic networks - and the problems caused by the complexity of intermediary metabolism in plants are discussed. It is concluded that metabolic flux analysis provides a powerful method for defining the metabolic phenotype of wild type, mutant and transgenic plants and that its development should be pursued. |
| spellingShingle | Kruger, N Ratcliffe, R Roscher, A Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling |
| title | Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling |
| title_full | Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling |
| title_fullStr | Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling |
| title_full_unstemmed | Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling |
| title_short | Quantitative approaches for analysing fluxes through plant metabolic networks using NMR and stable isotope labelling |
| title_sort | quantitative approaches for analysing fluxes through plant metabolic networks using nmr and stable isotope labelling |
| work_keys_str_mv | AT krugern quantitativeapproachesforanalysingfluxesthroughplantmetabolicnetworksusingnmrandstableisotopelabelling AT ratcliffer quantitativeapproachesforanalysingfluxesthroughplantmetabolicnetworksusingnmrandstableisotopelabelling AT roschera quantitativeapproachesforanalysingfluxesthroughplantmetabolicnetworksusingnmrandstableisotopelabelling |