Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects.
Accurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
American Society of Tropical Medicine and Hygiene
2015
|
| _version_ | 1797068156278145024 |
|---|---|
| author | Osorio, L Carter, N Arthur, P Bancone, G Gopalan, S Gupta, S Noedl, H Kochar, S Kochar, D Krudsood, S Lacerda, M Llanos-Cuentas, A Rueangweerayut, R Srinivasan, R Treiber, M Möhrle, J Green, J |
| author_facet | Osorio, L Carter, N Arthur, P Bancone, G Gopalan, S Gupta, S Noedl, H Kochar, S Kochar, D Krudsood, S Lacerda, M Llanos-Cuentas, A Rueangweerayut, R Srinivasan, R Treiber, M Möhrle, J Green, J |
| author_sort | Osorio, L |
| collection | OXFORD |
| description | Accurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in 356 Plasmodium vivax-infected subjects in Brazil, Peru, Thailand, and India. In the quantitative assay, the median G6PD activity was 8.81 U/g hemoglobin (range = 0.05-20.19), with 11 (3%) subjects identified as deficient. Sensitivity of the BinaxNOW G6PD to detect deficient subjects was 54.5% (6 of 11), and specificity was 100% (345 of 345). Room temperatures inadvertently falling outside the range required to perform the rapid test (18-25°C) together with subtlety of color change and insufficient training could partially explain the low sensitivity found. Ensuring safe use of 8-aminoquinolines depends on additional development of simple, highly sensitive G6PD deficiency diagnostic tests suitable for routine use in malaria-endemic areas. |
| first_indexed | 2024-03-06T22:06:38Z |
| format | Journal article |
| id | oxford-uuid:50651a49-7777-4e90-b6bf-881d13c823fd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:06:38Z |
| publishDate | 2015 |
| publisher | American Society of Tropical Medicine and Hygiene |
| record_format | dspace |
| spelling | oxford-uuid:50651a49-7777-4e90-b6bf-881d13c823fd2022-03-26T16:13:17ZPerformance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:50651a49-7777-4e90-b6bf-881d13c823fdEnglishSymplectic Elements at OxfordAmerican Society of Tropical Medicine and Hygiene2015Osorio, LCarter, NArthur, PBancone, GGopalan, SGupta, SNoedl, HKochar, SKochar, DKrudsood, SLacerda, MLlanos-Cuentas, ARueangweerayut, RSrinivasan, RTreiber, MMöhrle, JGreen, JAccurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in 356 Plasmodium vivax-infected subjects in Brazil, Peru, Thailand, and India. In the quantitative assay, the median G6PD activity was 8.81 U/g hemoglobin (range = 0.05-20.19), with 11 (3%) subjects identified as deficient. Sensitivity of the BinaxNOW G6PD to detect deficient subjects was 54.5% (6 of 11), and specificity was 100% (345 of 345). Room temperatures inadvertently falling outside the range required to perform the rapid test (18-25°C) together with subtlety of color change and insufficient training could partially explain the low sensitivity found. Ensuring safe use of 8-aminoquinolines depends on additional development of simple, highly sensitive G6PD deficiency diagnostic tests suitable for routine use in malaria-endemic areas. |
| spellingShingle | Osorio, L Carter, N Arthur, P Bancone, G Gopalan, S Gupta, S Noedl, H Kochar, S Kochar, D Krudsood, S Lacerda, M Llanos-Cuentas, A Rueangweerayut, R Srinivasan, R Treiber, M Möhrle, J Green, J Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects. |
| title | Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects. |
| title_full | Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects. |
| title_fullStr | Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects. |
| title_full_unstemmed | Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects. |
| title_short | Performance of BinaxNOW G6PD deficiency point-of-care diagnostic in P. vivax-infected subjects. |
| title_sort | performance of binaxnow g6pd deficiency point of care diagnostic in p vivax infected subjects |
| work_keys_str_mv | AT osoriol performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT cartern performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT arthurp performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT banconeg performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT gopalans performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT guptas performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT noedlh performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT kochars performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT kochard performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT krudsoods performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT lacerdam performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT llanoscuentasa performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT rueangweerayutr performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT srinivasanr performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT treiberm performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT mohrlej performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects AT greenj performanceofbinaxnowg6pddeficiencypointofcarediagnosticinpvivaxinfectedsubjects |