Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture
The establishment of a Plasmodium vivax in vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been l...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2012
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| _version_ | 1826272193012563968 |
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| author | Borlon, C Russell, B Sriprawat, K Suwanarusk, R Erhart, A Renia, L Nosten, F D'Alessandro, U |
| author_facet | Borlon, C Russell, B Sriprawat, K Suwanarusk, R Erhart, A Renia, L Nosten, F D'Alessandro, U |
| author_sort | Borlon, C |
| collection | OXFORD |
| description | The establishment of a Plasmodium vivax in vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been limited by the need for fresh parasite isolates. We explored the possibility of using parasite isolates and reticulocytes, both cryopreserved, to perform invasion and initiate short-term culture. Invasion results obtained with both cryopreserved isolates and reticulocytes were similar to those obtained with fresh samples. This method should be easily replicated in laboratories outside endemic areas and will substantially contribute to the development of a continuous P. vivax culture. In addition, this model could be used for testing vaccine candidates as well as for studying invasion-specific molecular mechanisms. © 2012 Australian Society for Parasitology Inc. |
| first_indexed | 2024-03-06T22:08:40Z |
| format | Journal article |
| id | oxford-uuid:510bf375-b0c8-4be6-8f86-b356481d12f1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:08:40Z |
| publishDate | 2012 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:510bf375-b0c8-4be6-8f86-b356481d12f12022-03-26T16:17:10ZCryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term cultureJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:510bf375-b0c8-4be6-8f86-b356481d12f1EnglishSymplectic Elements at OxfordElsevier2012Borlon, CRussell, BSriprawat, KSuwanarusk, RErhart, ARenia, LNosten, FD'Alessandro, UThe establishment of a Plasmodium vivax in vitro culture system is critical for the development of new vaccine, drugs and diagnostic tests. Although short-term cultures have been successfully set up, their reproducibility in laboratories without direct access to P. vivax-infected patients has been limited by the need for fresh parasite isolates. We explored the possibility of using parasite isolates and reticulocytes, both cryopreserved, to perform invasion and initiate short-term culture. Invasion results obtained with both cryopreserved isolates and reticulocytes were similar to those obtained with fresh samples. This method should be easily replicated in laboratories outside endemic areas and will substantially contribute to the development of a continuous P. vivax culture. In addition, this model could be used for testing vaccine candidates as well as for studying invasion-specific molecular mechanisms. © 2012 Australian Society for Parasitology Inc. |
| spellingShingle | Borlon, C Russell, B Sriprawat, K Suwanarusk, R Erhart, A Renia, L Nosten, F D'Alessandro, U Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
| title | Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
| title_full | Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
| title_fullStr | Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
| title_full_unstemmed | Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
| title_short | Cryopreserved Plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
| title_sort | cryopreserved plasmodium vivax and cord blood reticulocytes can be used for invasion and short term culture |
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