High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands.
We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful te...
Main Authors: | Tahiri-Alaoui, A, Frigotto, L, Manville, N, Ibrahim, J, Romby, P, James, W |
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Format: | Journal article |
Language: | English |
Published: |
2002
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