Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex.
The R1 plasmid employs ATP-driven polymerisation of the actin-like protein ParM to move newly replicated DNA to opposite poles of a bacterial cell. This process is essential for ensuring accurate segregation of the low-copy number plasmid and is the best characterised example of DNA partitioning in...
| Κύριοι συγγραφείς: | , |
|---|---|
| Μορφή: | Journal article |
| Γλώσσα: | English |
| Έκδοση: |
2008
|
| _version_ | 1826272298211999744 |
|---|---|
| author | Salje, J Löwe, J |
| author_facet | Salje, J Löwe, J |
| author_sort | Salje, J |
| collection | OXFORD |
| description | The R1 plasmid employs ATP-driven polymerisation of the actin-like protein ParM to move newly replicated DNA to opposite poles of a bacterial cell. This process is essential for ensuring accurate segregation of the low-copy number plasmid and is the best characterised example of DNA partitioning in prokaryotes. In vivo, ParM only forms long filaments when capped at both ends by attachment to a centromere-like region parC, through a small DNA-binding protein ParR. Here, we present biochemical and electron microscopy data leading to a model for the mechanism by which ParR-parC complexes bind and stabilise elongating ParM filaments. We propose that the open ring formed by oligomeric ParR dimers with parC DNA wrapped around acts as a rigid clamp, which holds the end of elongating ParM filaments while allowing entry of new ATP-bound monomers. We propose a processive mechanism by which cycles of ATP hydrolysis in polymerising ParM drives movement of ParR-bound parC DNA. Importantly, our model predicts that each pair of plasmids will be driven apart in the cell by just a single double helical ParM filament. |
| first_indexed | 2024-03-06T22:10:20Z |
| format | Journal article |
| id | oxford-uuid:5197112b-e32c-4e49-bfd4-908719cee587 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:10:20Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:5197112b-e32c-4e49-bfd4-908719cee5872022-03-26T16:20:28ZBacterial actin: architecture of the ParMRC plasmid DNA partitioning complex.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5197112b-e32c-4e49-bfd4-908719cee587EnglishSymplectic Elements at Oxford2008Salje, JLöwe, JThe R1 plasmid employs ATP-driven polymerisation of the actin-like protein ParM to move newly replicated DNA to opposite poles of a bacterial cell. This process is essential for ensuring accurate segregation of the low-copy number plasmid and is the best characterised example of DNA partitioning in prokaryotes. In vivo, ParM only forms long filaments when capped at both ends by attachment to a centromere-like region parC, through a small DNA-binding protein ParR. Here, we present biochemical and electron microscopy data leading to a model for the mechanism by which ParR-parC complexes bind and stabilise elongating ParM filaments. We propose that the open ring formed by oligomeric ParR dimers with parC DNA wrapped around acts as a rigid clamp, which holds the end of elongating ParM filaments while allowing entry of new ATP-bound monomers. We propose a processive mechanism by which cycles of ATP hydrolysis in polymerising ParM drives movement of ParR-bound parC DNA. Importantly, our model predicts that each pair of plasmids will be driven apart in the cell by just a single double helical ParM filament. |
| spellingShingle | Salje, J Löwe, J Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex. |
| title | Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex. |
| title_full | Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex. |
| title_fullStr | Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex. |
| title_full_unstemmed | Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex. |
| title_short | Bacterial actin: architecture of the ParMRC plasmid DNA partitioning complex. |
| title_sort | bacterial actin architecture of the parmrc plasmid dna partitioning complex |
| work_keys_str_mv | AT saljej bacterialactinarchitectureoftheparmrcplasmiddnapartitioningcomplex AT lowej bacterialactinarchitectureoftheparmrcplasmiddnapartitioningcomplex |