Dynamic imaging of the effector immune response to listeria infection in vivo.
Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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| _version_ | 1797068474866991104 |
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| author | Waite, J Leiner, I Lauer, P Rae, C Barbet, G Zheng, H Portnoy, D Pamer, E Dustin, M |
| author_facet | Waite, J Leiner, I Lauer, P Rae, C Barbet, G Zheng, H Portnoy, D Pamer, E Dustin, M |
| author_sort | Waite, J |
| collection | OXFORD |
| description | Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30-1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8+ T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8+ T cells in effector sites. |
| first_indexed | 2024-03-06T22:11:15Z |
| format | Journal article |
| id | oxford-uuid:51e03de4-6bfb-410d-b07b-37c6381709e3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:11:15Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:51e03de4-6bfb-410d-b07b-37c6381709e32022-03-26T16:22:18ZDynamic imaging of the effector immune response to listeria infection in vivo.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:51e03de4-6bfb-410d-b07b-37c6381709e3EnglishSymplectic Elements at Oxford2011Waite, JLeiner, ILauer, PRae, CBarbet, GZheng, HPortnoy, DPamer, EDustin, MHost defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30-1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8+ T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8+ T cells in effector sites. |
| spellingShingle | Waite, J Leiner, I Lauer, P Rae, C Barbet, G Zheng, H Portnoy, D Pamer, E Dustin, M Dynamic imaging of the effector immune response to listeria infection in vivo. |
| title | Dynamic imaging of the effector immune response to listeria infection in vivo. |
| title_full | Dynamic imaging of the effector immune response to listeria infection in vivo. |
| title_fullStr | Dynamic imaging of the effector immune response to listeria infection in vivo. |
| title_full_unstemmed | Dynamic imaging of the effector immune response to listeria infection in vivo. |
| title_short | Dynamic imaging of the effector immune response to listeria infection in vivo. |
| title_sort | dynamic imaging of the effector immune response to listeria infection in vivo |
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