Tandem affinity purification of functional TAP-tagged proteins from human cells.
Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome to heterologously express TAP-tagged murine Sgo1 protein in human HeLa cells. This allowed us to tes...
Повний опис
Бібліографічні деталі
| Автори: |
Gregan, J,
Riedel, C,
Petronczki, M,
Cipak, L,
Rumpf, C,
Poser, I,
Buchholz, F,
Mechtler, K,
Nasmyth, K |
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| Формат: | Journal article
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| Мова: | English |
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| Опубліковано: |
2007
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