Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan.
In skeletal muscle, the cytolinker plectin is prominently expressed at Z-disks and the sarcolemma. Alternative splicing of plectin transcripts gives rise to more than eight protein isoforms differing only in small N-terminal sequences (5-180 residues), four of which (plectins 1, 1b, 1d, and 1f) are...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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| _version_ | 1797068612943478784 |
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| author | Rezniczek, G Konieczny, P Nikolic, B Reipert, S Schneller, D Abrahamsberg, C Davies, K Winder, S Wiche, G |
| author_facet | Rezniczek, G Konieczny, P Nikolic, B Reipert, S Schneller, D Abrahamsberg, C Davies, K Winder, S Wiche, G |
| author_sort | Rezniczek, G |
| collection | OXFORD |
| description | In skeletal muscle, the cytolinker plectin is prominently expressed at Z-disks and the sarcolemma. Alternative splicing of plectin transcripts gives rise to more than eight protein isoforms differing only in small N-terminal sequences (5-180 residues), four of which (plectins 1, 1b, 1d, and 1f) are found at substantial levels in muscle tissue. Using plectin isoform-specific antibodies and isoform expression constructs, we show the differential regulation of plectin isoforms during myotube differentiation and their localization to different compartments of muscle fibers, identifying plectins 1 and 1f as sarcolemma-associated isoforms, whereas plectin 1d localizes exclusively to Z-disks. Coimmunoprecipitation and in vitro binding assays using recombinant protein fragments revealed the direct binding of plectin to dystrophin (utrophin) and beta-dystroglycan, the key components of the dystrophin-glycoprotein complex. We propose a model in which plectin acts as a universal mediator of desmin intermediate filament anchorage at the sarcolemma and Z-disks. It also explains the plectin phenotype observed in dystrophic skeletal muscle of mdx mice and Duchenne muscular dystrophy patients. |
| first_indexed | 2024-03-06T22:12:40Z |
| format | Journal article |
| id | oxford-uuid:52564be7-bb5b-4279-9a75-0fa65115bf8c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:12:40Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:52564be7-bb5b-4279-9a75-0fa65115bf8c2022-03-26T16:25:03ZPlectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:52564be7-bb5b-4279-9a75-0fa65115bf8cEnglishSymplectic Elements at Oxford2007Rezniczek, GKonieczny, PNikolic, BReipert, SSchneller, DAbrahamsberg, CDavies, KWinder, SWiche, GIn skeletal muscle, the cytolinker plectin is prominently expressed at Z-disks and the sarcolemma. Alternative splicing of plectin transcripts gives rise to more than eight protein isoforms differing only in small N-terminal sequences (5-180 residues), four of which (plectins 1, 1b, 1d, and 1f) are found at substantial levels in muscle tissue. Using plectin isoform-specific antibodies and isoform expression constructs, we show the differential regulation of plectin isoforms during myotube differentiation and their localization to different compartments of muscle fibers, identifying plectins 1 and 1f as sarcolemma-associated isoforms, whereas plectin 1d localizes exclusively to Z-disks. Coimmunoprecipitation and in vitro binding assays using recombinant protein fragments revealed the direct binding of plectin to dystrophin (utrophin) and beta-dystroglycan, the key components of the dystrophin-glycoprotein complex. We propose a model in which plectin acts as a universal mediator of desmin intermediate filament anchorage at the sarcolemma and Z-disks. It also explains the plectin phenotype observed in dystrophic skeletal muscle of mdx mice and Duchenne muscular dystrophy patients. |
| spellingShingle | Rezniczek, G Konieczny, P Nikolic, B Reipert, S Schneller, D Abrahamsberg, C Davies, K Winder, S Wiche, G Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. |
| title | Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. |
| title_full | Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. |
| title_fullStr | Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. |
| title_full_unstemmed | Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. |
| title_short | Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. |
| title_sort | plectin 1f scaffolding at the sarcolemma of dystrophic mdx muscle fibers through multiple interactions with beta dystroglycan |
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