Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain
Epigenetic processes play a key role in the central nervous system and altered levels of 5-methylcytosine have been associated with a number of neurologic phenotypes, including Alzheimer's disease (AD). Recently, 3 additional cytosine modifications have been identified (5-hydroxymethylcytosine,...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
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2014
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| _version_ | 1797068636838428672 |
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| author | Condliffe, D Condliffe, D Wong, A Troakes, C Proitsi, P Patel, Y Chouliaras, L Fernandes, C Cooper, J Lovestone, S Schalkwyk, L Mill, J Mill, J Lunnon, K |
| author_facet | Condliffe, D Condliffe, D Wong, A Troakes, C Proitsi, P Patel, Y Chouliaras, L Fernandes, C Cooper, J Lovestone, S Schalkwyk, L Mill, J Mill, J Lunnon, K |
| author_sort | Condliffe, D |
| collection | OXFORD |
| description | Epigenetic processes play a key role in the central nervous system and altered levels of 5-methylcytosine have been associated with a number of neurologic phenotypes, including Alzheimer's disease (AD). Recently, 3 additional cytosine modifications have been identified (5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine), which are thought to be intermediate steps in the demethylation of 5-methylcytosine to unmodified cytosine. Little is known about the frequency of these modifications in the human brain during health or disease. In this study, we used immunofluorescence to confirm the presence of each modification in human brain and investigate their cross-tissue abundance in AD patients and elderly control samples. We identify a significant AD-associated decrease in global 5-hydroxymethylcytosine in entorhinal cortex and cerebellum, and differences in 5-formylcytosine levels between brain regions. Our study further implicates a role for epigenetic alterations in AD. © 2014 Elsevier Inc. |
| first_indexed | 2024-03-06T22:13:01Z |
| format | Journal article |
| id | oxford-uuid:52715e7d-6fa7-42ec-af40-c293b2efc7f9 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T22:13:01Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:52715e7d-6fa7-42ec-af40-c293b2efc7f92022-03-26T16:25:46ZCross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brainJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:52715e7d-6fa7-42ec-af40-c293b2efc7f9Symplectic Elements at Oxford2014Condliffe, DCondliffe, DWong, ATroakes, CProitsi, PPatel, YChouliaras, LFernandes, CCooper, JLovestone, SSchalkwyk, LMill, JMill, JLunnon, KEpigenetic processes play a key role in the central nervous system and altered levels of 5-methylcytosine have been associated with a number of neurologic phenotypes, including Alzheimer's disease (AD). Recently, 3 additional cytosine modifications have been identified (5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine), which are thought to be intermediate steps in the demethylation of 5-methylcytosine to unmodified cytosine. Little is known about the frequency of these modifications in the human brain during health or disease. In this study, we used immunofluorescence to confirm the presence of each modification in human brain and investigate their cross-tissue abundance in AD patients and elderly control samples. We identify a significant AD-associated decrease in global 5-hydroxymethylcytosine in entorhinal cortex and cerebellum, and differences in 5-formylcytosine levels between brain regions. Our study further implicates a role for epigenetic alterations in AD. © 2014 Elsevier Inc. |
| spellingShingle | Condliffe, D Condliffe, D Wong, A Troakes, C Proitsi, P Patel, Y Chouliaras, L Fernandes, C Cooper, J Lovestone, S Schalkwyk, L Mill, J Mill, J Lunnon, K Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain |
| title | Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain |
| title_full | Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain |
| title_fullStr | Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain |
| title_full_unstemmed | Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain |
| title_short | Cross-region reduction in 5-hydroxymethylcytosine in Alzheimer's disease brain |
| title_sort | cross region reduction in 5 hydroxymethylcytosine in alzheimer s disease brain |
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