A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids.
Cell-penetrating peptides (CPPs) have attracted increasing attention in the past decade as a result of their high potential to convey various, otherwise impermeable, bioactive agents across cellular plasma membranes. Albeit different CPPs have proven potent in delivery of different cargoes, there is...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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| _version_ | 1797068758297083904 |
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| author | El-Andaloussi, S Johansson, H Holm, T Langel, U |
| author_facet | El-Andaloussi, S Johansson, H Holm, T Langel, U |
| author_sort | El-Andaloussi, S |
| collection | OXFORD |
| description | Cell-penetrating peptides (CPPs) have attracted increasing attention in the past decade as a result of their high potential to convey various, otherwise impermeable, bioactive agents across cellular plasma membranes. Albeit different CPPs have proven potent in delivery of different cargoes, there is generally a correlation between high efficacy and cytotoxicity for these peptides. Hence, it is of great importance to find new, non-toxic CPPs with more widespread delivery properties. We present a novel CPP, M918, that efficiently translocates various cells in a non-toxic fashion. In line with most other CPPs, the peptide is internalized mainly via endocytosis, and in particular macropinocytosis, but independent of glycosaminoglycans on the cell surface. In addition, in a splice correction assay using antisense peptide nucleic acid (PNA) conjugated via a disulphide bridge to M918 (M918-PNA), we observed a dose-dependent increase in correct splicing, exceeding the effect of other CPPs. Our data demonstrate that M918 is a novel CPP that can be used to translocate different cargoes inside various cells efficiently. |
| first_indexed | 2024-03-06T22:14:42Z |
| format | Journal article |
| id | oxford-uuid:52ffc8b8-8782-4ce9-b19d-d16c396e8fb8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:14:42Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:52ffc8b8-8782-4ce9-b19d-d16c396e8fb82022-03-26T16:28:56ZA novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:52ffc8b8-8782-4ce9-b19d-d16c396e8fb8EnglishSymplectic Elements at Oxford2007El-Andaloussi, SJohansson, HHolm, TLangel, UCell-penetrating peptides (CPPs) have attracted increasing attention in the past decade as a result of their high potential to convey various, otherwise impermeable, bioactive agents across cellular plasma membranes. Albeit different CPPs have proven potent in delivery of different cargoes, there is generally a correlation between high efficacy and cytotoxicity for these peptides. Hence, it is of great importance to find new, non-toxic CPPs with more widespread delivery properties. We present a novel CPP, M918, that efficiently translocates various cells in a non-toxic fashion. In line with most other CPPs, the peptide is internalized mainly via endocytosis, and in particular macropinocytosis, but independent of glycosaminoglycans on the cell surface. In addition, in a splice correction assay using antisense peptide nucleic acid (PNA) conjugated via a disulphide bridge to M918 (M918-PNA), we observed a dose-dependent increase in correct splicing, exceeding the effect of other CPPs. Our data demonstrate that M918 is a novel CPP that can be used to translocate different cargoes inside various cells efficiently. |
| spellingShingle | El-Andaloussi, S Johansson, H Holm, T Langel, U A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids. |
| title | A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids. |
| title_full | A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids. |
| title_fullStr | A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids. |
| title_full_unstemmed | A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids. |
| title_short | A novel cell-penetrating peptide, M918, for efficient delivery of proteins and peptide nucleic acids. |
| title_sort | novel cell penetrating peptide m918 for efficient delivery of proteins and peptide nucleic acids |
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