Ultrastructural investigation methods for Trypanosoma brucei.
Trypanosoma brucei is a unicellular parasite causing African sleeping sickness in cattle and humans. Due to the ease with which these cells can be cultured and genetically manipulated, it has emerged as a model organism for the kinetoplastids.In this chapter we describe the preparation of T. brucei...
| Auteurs principaux: | , , , |
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| Format: | Journal article |
| Langue: | English |
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2010
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| _version_ | 1826272706098626560 |
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| author | Höög, J Gluenz, E Vaughan, S Gull, K |
| author_facet | Höög, J Gluenz, E Vaughan, S Gull, K |
| author_sort | Höög, J |
| collection | OXFORD |
| description | Trypanosoma brucei is a unicellular parasite causing African sleeping sickness in cattle and humans. Due to the ease with which these cells can be cultured and genetically manipulated, it has emerged as a model organism for the kinetoplastids.In this chapter we describe the preparation of T. brucei for transmission electron microscopy. A thorough explanation of conventional sample preparation through chemical fixation of whole cells and detergent extracted cytoskeletons followed by dehydration and Epon embedding is given. We also introduce a novel high-pressure freezing protocol, which followed by rapid freeze substitution and HM20 embedding generates T. brucei samples displaying good cell morphology, which are suitable for immunocytochemistry. |
| first_indexed | 2024-03-06T22:16:49Z |
| format | Journal article |
| id | oxford-uuid:53a64c75-dd34-4a15-a8c2-cb41ed18f9a7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:16:49Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:53a64c75-dd34-4a15-a8c2-cb41ed18f9a72022-03-26T16:33:04ZUltrastructural investigation methods for Trypanosoma brucei.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:53a64c75-dd34-4a15-a8c2-cb41ed18f9a7EnglishSymplectic Elements at Oxford2010Höög, JGluenz, EVaughan, SGull, KTrypanosoma brucei is a unicellular parasite causing African sleeping sickness in cattle and humans. Due to the ease with which these cells can be cultured and genetically manipulated, it has emerged as a model organism for the kinetoplastids.In this chapter we describe the preparation of T. brucei for transmission electron microscopy. A thorough explanation of conventional sample preparation through chemical fixation of whole cells and detergent extracted cytoskeletons followed by dehydration and Epon embedding is given. We also introduce a novel high-pressure freezing protocol, which followed by rapid freeze substitution and HM20 embedding generates T. brucei samples displaying good cell morphology, which are suitable for immunocytochemistry. |
| spellingShingle | Höög, J Gluenz, E Vaughan, S Gull, K Ultrastructural investigation methods for Trypanosoma brucei. |
| title | Ultrastructural investigation methods for Trypanosoma brucei. |
| title_full | Ultrastructural investigation methods for Trypanosoma brucei. |
| title_fullStr | Ultrastructural investigation methods for Trypanosoma brucei. |
| title_full_unstemmed | Ultrastructural investigation methods for Trypanosoma brucei. |
| title_short | Ultrastructural investigation methods for Trypanosoma brucei. |
| title_sort | ultrastructural investigation methods for trypanosoma brucei |
| work_keys_str_mv | AT hoogj ultrastructuralinvestigationmethodsfortrypanosomabrucei AT gluenze ultrastructuralinvestigationmethodsfortrypanosomabrucei AT vaughans ultrastructuralinvestigationmethodsfortrypanosomabrucei AT gullk ultrastructuralinvestigationmethodsfortrypanosomabrucei |