A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.
Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designa...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1979
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| _version_ | 1826272833626439680 |
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| author | Mcmichael, A Pilch, JR Galfré, G Mason, D Fabre, J Milstein, C |
| author_facet | Mcmichael, A Pilch, JR Galfré, G Mason, D Fabre, J Milstein, C |
| author_sort | Mcmichael, A |
| collection | OXFORD |
| description | Spleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight. |
| first_indexed | 2024-03-06T22:18:50Z |
| format | Journal article |
| id | oxford-uuid:54546e98-3f84-455c-9af0-4392a6bd77e5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:18:50Z |
| publishDate | 1979 |
| record_format | dspace |
| spelling | oxford-uuid:54546e98-3f84-455c-9af0-4392a6bd77e52022-03-26T16:37:12ZA human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:54546e98-3f84-455c-9af0-4392a6bd77e5EnglishSymplectic Elements at Oxford1979Mcmichael, APilch, JRGalfré, GMason, DFabre, JMilstein, CSpleen cells from a BALB/c mouse that had been immunized with human thymocytes were fused with the myeloma line P3-NS 1/1 Ag 4.1. One of the resulting hybrid clones (NA 1/34) secreted an antibody that was highly specific for human thymocytes. Eighty-five % of thymocytes expressed the antigen designated HTA1. There were an estimated 15 x 10(4) molecules of HTA 1 per cell, and it is therefore a major surface molecule. The expression of this antigen on thymocytes appears to be reciprocal to HLA, as recognized by another monoclonal antibody W6/32. Immunoprecipitated material from [125I]-labeled thymocyte membranes was analyzed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate which disclosed a single component of 45,000 molecular weight. |
| spellingShingle | Mcmichael, A Pilch, JR Galfré, G Mason, D Fabre, J Milstein, C A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. |
| title | A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. |
| title_full | A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. |
| title_fullStr | A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. |
| title_full_unstemmed | A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. |
| title_short | A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. |
| title_sort | human thymocyte antigen defined by a hybrid myeloma monoclonal antibody |
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