Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe.
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed in Saccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate reductase, to the N-terminus of the target protein, which there...
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Bibliographic Details
| Main Authors: |
Kearsey, S,
Gregan, J |
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| Format: | Journal article
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| Language: | English |
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| Published: |
2009
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