Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe.
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed in Saccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate reductase, to the N-terminus of the target protein, which there...
| Main Authors: | , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2009
|
| _version_ | 1826272913822580736 |
|---|---|
| author | Kearsey, S Gregan, J |
| author_facet | Kearsey, S Gregan, J |
| author_sort | Kearsey, S |
| collection | OXFORD |
| description | Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed in Saccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate reductase, to the N-terminus of the target protein, which thereby confers temperature-sensitive degradation at the restrictive temperature. We describe here the application of this technique in the fission yeast, Schizosaccharomyces pombe. |
| first_indexed | 2024-03-06T22:20:05Z |
| format | Journal article |
| id | oxford-uuid:54b99013-523c-4b70-b19e-440074a298b3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:20:05Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:54b99013-523c-4b70-b19e-440074a298b32022-03-26T16:39:45ZUsing the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:54b99013-523c-4b70-b19e-440074a298b3EnglishSymplectic Elements at Oxford2009Kearsey, SGregan, JInactivating a specific protein in vivo can yield important information about its function. One strategy previously developed in Saccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate reductase, to the N-terminus of the target protein, which thereby confers temperature-sensitive degradation at the restrictive temperature. We describe here the application of this technique in the fission yeast, Schizosaccharomyces pombe. |
| spellingShingle | Kearsey, S Gregan, J Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe. |
| title | Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe. |
| title_full | Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe. |
| title_fullStr | Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe. |
| title_full_unstemmed | Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe. |
| title_short | Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe. |
| title_sort | using the dhfr heat inducible degron for protein inactivation in schizosaccharomyces pombe |
| work_keys_str_mv | AT kearseys usingthedhfrheatinducibledegronforproteininactivationinschizosaccharomycespombe AT greganj usingthedhfrheatinducibledegronforproteininactivationinschizosaccharomycespombe |