Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancer
The development of new sensors for the accurate detection of biomarkers in biological fluids is of utmost importance for the early diagnosis of diseases. Next to advanced laboratory techniques, there is a need for relatively simple methods which can significantly broaden the availability of diagnost...
| Main Authors: | , , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
American Chemical Society
2016
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| _version_ | 1826272991537790976 |
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| author | Vilela, P El-Sagheer, A Millar, T Brown, T Muskens, O Kanaras, A |
| author_facet | Vilela, P El-Sagheer, A Millar, T Brown, T Muskens, O Kanaras, A |
| author_sort | Vilela, P |
| collection | OXFORD |
| description | The development of new sensors for the accurate detection of biomarkers in biological fluids is of utmost importance for the early diagnosis of diseases. Next to advanced laboratory techniques, there is a need for relatively simple methods which can significantly broaden the availability of diagnostic capability. Here, we demonstrate the successful application of a sensor platform based on graphene oxide and upconversion nanoparticles (NPs) for the specific detection of mRNA-related oligonucleotide markers in complex biological fluids. The combination of near-infrared light upconversion with low-background photon counting readout enables reliable detection of low quantities of small oligonucleotide sequences in the femtomolar range. We demonstrate the successful detection of analytes relevant to mRNAs present in Alzheimer's disease as well as prostate cancer in human blood serum. The high performance and relative simplicity of the upconversion NP-graphene sensor platform enables new opportunities in early diagnosis based on specific detection of oligonucleotide sequences in complex environments. |
| first_indexed | 2024-03-06T22:21:20Z |
| format | Journal article |
| id | oxford-uuid:5527967d-c94e-4f10-9c6e-b7504c514b57 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:21:20Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:5527967d-c94e-4f10-9c6e-b7504c514b572022-03-26T16:42:21ZGraphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancerJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5527967d-c94e-4f10-9c6e-b7504c514b57EnglishSymplectic Elements at OxfordAmerican Chemical Society2016Vilela, PEl-Sagheer, AMillar, TBrown, TMuskens, OKanaras, AThe development of new sensors for the accurate detection of biomarkers in biological fluids is of utmost importance for the early diagnosis of diseases. Next to advanced laboratory techniques, there is a need for relatively simple methods which can significantly broaden the availability of diagnostic capability. Here, we demonstrate the successful application of a sensor platform based on graphene oxide and upconversion nanoparticles (NPs) for the specific detection of mRNA-related oligonucleotide markers in complex biological fluids. The combination of near-infrared light upconversion with low-background photon counting readout enables reliable detection of low quantities of small oligonucleotide sequences in the femtomolar range. We demonstrate the successful detection of analytes relevant to mRNAs present in Alzheimer's disease as well as prostate cancer in human blood serum. The high performance and relative simplicity of the upconversion NP-graphene sensor platform enables new opportunities in early diagnosis based on specific detection of oligonucleotide sequences in complex environments. |
| spellingShingle | Vilela, P El-Sagheer, A Millar, T Brown, T Muskens, O Kanaras, A Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancer |
| title | Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancer |
| title_full | Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancer |
| title_fullStr | Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancer |
| title_full_unstemmed | Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancer |
| title_short | Graphene oxide-upconversion nanoparticle based optical sensors for targeted detection of mRNA biomarkers present in alzheimer's disease and prostate cancer |
| title_sort | graphene oxide upconversion nanoparticle based optical sensors for targeted detection of mrna biomarkers present in alzheimer s disease and prostate cancer |
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