Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae.
The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [l-α-d...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1797069304761417728 |
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| author | Twelkmeyer, B Burström, P Li, J Richard Moxon, E Hood, D Schweda, E |
| author_facet | Twelkmeyer, B Burström, P Li, J Richard Moxon, E Hood, D Schweda, E |
| author_sort | Twelkmeyer, B |
| collection | OXFORD |
| description | The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [l-α-d-Hepp-(1→2)-[PEtn→6]-l-α-d-Hepp-(1→3)-l-α-d-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-d-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-d-GalpNAc-(1→3)-α-d-Galp-(1→4)-β-d-Galp-(1→4)-β-d-Glcp-(1→] and its truncated versions globoside [α-d-Galp-(1→4)-β-d-Galp-(1→4)-β-d-Glcp-(1→] and lactose [β-d-Galp-(1→4)-β-d-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS. |
| first_indexed | 2024-03-06T22:22:28Z |
| format | Journal article |
| id | oxford-uuid:5585a344-d47a-4398-aef6-bba027b488fe |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:22:28Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:5585a344-d47a-4398-aef6-bba027b488fe2022-03-26T16:44:29ZExpression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5585a344-d47a-4398-aef6-bba027b488feEnglishSymplectic Elements at Oxford2011Twelkmeyer, BBurström, PLi, JRichard Moxon, EHood, DSchweda, EThe structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [l-α-d-Hepp-(1→2)-[PEtn→6]-l-α-d-Hepp-(1→3)-l-α-d-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-d-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-d-GalpNAc-(1→3)-α-d-Galp-(1→4)-β-d-Galp-(1→4)-β-d-Glcp-(1→] and its truncated versions globoside [α-d-Galp-(1→4)-β-d-Galp-(1→4)-β-d-Glcp-(1→] and lactose [β-d-Galp-(1→4)-β-d-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS. |
| spellingShingle | Twelkmeyer, B Burström, P Li, J Richard Moxon, E Hood, D Schweda, E Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae. |
| title | Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae. |
| title_full | Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae. |
| title_fullStr | Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae. |
| title_full_unstemmed | Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae. |
| title_short | Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae. |
| title_sort | expression of a new disialyllacto structure in the lipopolysaccharide of non typeable haemophilus influenzae |
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