Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019
<p><strong>BACKGROUND:</strong> Despite efforts to mitigate the impact of DENV epidemics, the virus remains a public health problem in tropical and subtropical regions around the world. Most DENV cases in the Americas between January and July 2019 were reported in Brazil. São Paulo...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2020
|
| _version_ | 1797069333666463744 |
|---|---|
| author | Goes de Jesus, J Rocha Dutra, K da Silva Sales, FC Morales Claro, I Terzian, AC da Silva Candido, D Hill, S Theze, J Torres, C Lang D'Agostini, T Felix, AC Negri Reis, AF Alcantara Jr., AC de Abreu, AL Croda, JHR de Oliveira, WK Bispo de Filipis, AM do Carmo Rodrigues dos Santon Camis, M Malta-Romano, C Loman, NJ Pybus, OG Cerdeira Sabino, E Nogueira, ML Faria, N |
| author_facet | Goes de Jesus, J Rocha Dutra, K da Silva Sales, FC Morales Claro, I Terzian, AC da Silva Candido, D Hill, S Theze, J Torres, C Lang D'Agostini, T Felix, AC Negri Reis, AF Alcantara Jr., AC de Abreu, AL Croda, JHR de Oliveira, WK Bispo de Filipis, AM do Carmo Rodrigues dos Santon Camis, M Malta-Romano, C Loman, NJ Pybus, OG Cerdeira Sabino, E Nogueira, ML Faria, N |
| author_sort | Goes de Jesus, J |
| collection | OXFORD |
| description | <p><strong>BACKGROUND:</strong> Despite efforts to mitigate the impact of DENV epidemics, the virus remains a public health problem in tropical and subtropical regions around the world. Most DENV cases in the Americas between January and July 2019 were reported in Brazil. São Paulo state in the southeast of Brazil has reported nearly half of all DENV infections in the country.</p>
<p><strong>OBJECTIVES:</strong> To understand the origin and dynamics of the 2019 DENV outbreak.</p>
<p><strong>METHODS:</strong> Here using portable nanopore sequencing we generated 20 new DENV genome sequences from viremic patients with suspected dengue infection residing in two of the most-affected municipalities of Sao Paulo state, Araraquara and São José do Rio Preto. We conducted a comprehensive phylogenetic analysis with 1,630 global DENV strains to better understand the evolutionary history of the DENV lineages that currently circulate in the region.</p>
<p><strong>FINDINGS:</strong> The new outbreak strains were classified as DENV2 genotype III (American/Asian genotype). Our analysis shows that the 2019 outbreak is the result of a novel DENV lineage that was recently introduced to Brazil from the Caribbean region. Dating phylogeographic analysis suggests that DENV2-III BR-4 was introduced to Brazil in or around early 2014, possibly from the Caribbean region.</p>
<p><strong>MAIN CONCLUSIONS:</strong> Our study describes the early detection of a newly introduced and rapidly-expanding DENV2 virus lineage in Brazil.</p> |
| first_indexed | 2024-03-06T22:22:53Z |
| format | Journal article |
| id | oxford-uuid:55abad9f-89a6-44bd-b1f8-0392c3e936f4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:22:53Z |
| publishDate | 2020 |
| publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
| record_format | dspace |
| spelling | oxford-uuid:55abad9f-89a6-44bd-b1f8-0392c3e936f42022-03-26T16:45:34ZGenomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:55abad9f-89a6-44bd-b1f8-0392c3e936f4EnglishSymplectic ElementsInstituto Oswaldo Cruz, Ministério da Saúde2020Goes de Jesus, JRocha Dutra, Kda Silva Sales, FCMorales Claro, ITerzian, ACda Silva Candido, DHill, STheze, JTorres, CLang D'Agostini, TFelix, ACNegri Reis, AFAlcantara Jr., ACde Abreu, ALCroda, JHRde Oliveira, WKBispo de Filipis, AMdo Carmo Rodrigues dos Santon Camis, MMalta-Romano, CLoman, NJPybus, OGCerdeira Sabino, ENogueira, MLFaria, N<p><strong>BACKGROUND:</strong> Despite efforts to mitigate the impact of DENV epidemics, the virus remains a public health problem in tropical and subtropical regions around the world. Most DENV cases in the Americas between January and July 2019 were reported in Brazil. São Paulo state in the southeast of Brazil has reported nearly half of all DENV infections in the country.</p> <p><strong>OBJECTIVES:</strong> To understand the origin and dynamics of the 2019 DENV outbreak.</p> <p><strong>METHODS:</strong> Here using portable nanopore sequencing we generated 20 new DENV genome sequences from viremic patients with suspected dengue infection residing in two of the most-affected municipalities of Sao Paulo state, Araraquara and São José do Rio Preto. We conducted a comprehensive phylogenetic analysis with 1,630 global DENV strains to better understand the evolutionary history of the DENV lineages that currently circulate in the region.</p> <p><strong>FINDINGS:</strong> The new outbreak strains were classified as DENV2 genotype III (American/Asian genotype). Our analysis shows that the 2019 outbreak is the result of a novel DENV lineage that was recently introduced to Brazil from the Caribbean region. Dating phylogeographic analysis suggests that DENV2-III BR-4 was introduced to Brazil in or around early 2014, possibly from the Caribbean region.</p> <p><strong>MAIN CONCLUSIONS:</strong> Our study describes the early detection of a newly introduced and rapidly-expanding DENV2 virus lineage in Brazil.</p> |
| spellingShingle | Goes de Jesus, J Rocha Dutra, K da Silva Sales, FC Morales Claro, I Terzian, AC da Silva Candido, D Hill, S Theze, J Torres, C Lang D'Agostini, T Felix, AC Negri Reis, AF Alcantara Jr., AC de Abreu, AL Croda, JHR de Oliveira, WK Bispo de Filipis, AM do Carmo Rodrigues dos Santon Camis, M Malta-Romano, C Loman, NJ Pybus, OG Cerdeira Sabino, E Nogueira, ML Faria, N Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019 |
| title | Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019 |
| title_full | Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019 |
| title_fullStr | Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019 |
| title_full_unstemmed | Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019 |
| title_short | Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019 |
| title_sort | genomic detection of a virus lineage replacement event of dengue virus serotype 2 in brazil 2019 |
| work_keys_str_mv | AT goesdejesusj genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT rochadutrak genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT dasilvasalesfc genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT moralesclaroi genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT terzianac genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT dasilvacandidod genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT hills genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT thezej genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT torresc genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT langdagostinit genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT felixac genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT negrireisaf genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT alcantarajrac genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT deabreual genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT crodajhr genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT deoliveirawk genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT bispodefilipisam genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT docarmorodriguesdossantoncamism genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT maltaromanoc genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT lomannj genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT pybusog genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT cerdeirasabinoe genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT nogueiraml genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 AT farian genomicdetectionofaviruslineagereplacementeventofdenguevirusserotype2inbrazil2019 |