Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase.
We demonstrate a novel in situ infrared spectroscopic approach for the characterization of hydrogenase during catalytic turnover. E. coli hydrogenase 1 (Hyd-1) is adsorbed on a high surface-area carbon electrode and subjected to the same electrochemical control and efficient supply of substrate as i...
| Päätekijät: | , , , |
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| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
Wiley
2015
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| _version_ | 1826273119077138432 |
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| author | Ash, P Healy, A Vincent, K Hidalgo Gonzalez, R |
| author_facet | Ash, P Healy, A Vincent, K Hidalgo Gonzalez, R |
| author_sort | Ash, P |
| collection | OXFORD |
| description | We demonstrate a novel in situ infrared spectroscopic approach for the characterization of hydrogenase during catalytic turnover. E. coli hydrogenase 1 (Hyd-1) is adsorbed on a high surface-area carbon electrode and subjected to the same electrochemical control and efficient supply of substrate as in protein film electrochemistry during spectral acquisition. The spectra reveal that the active site state known as Ni-L, observed in other NiFe hydrogenases only under illumination or at cryogenic temperatures, can be generated reversibly in the dark at ambient temperature under both turnover and non-turnover conditions. The observation that Ni-L is present at all potentials during turnover under H2, suggests that the final steps in the catalytic cycle of H2 oxidation by Hyd-1 involve sequential proton- and electron-transfer via Ni-L. We present a broadly applicable IR spectroscopic technique for addressing electrode-adsorbed redox enzymes under fast catalytic turnover. |
| first_indexed | 2024-03-06T22:23:19Z |
| format | Journal article |
| id | oxford-uuid:55cd0288-01a1-4f5c-9f9a-f35d5ff22978 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:23:19Z |
| publishDate | 2015 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:55cd0288-01a1-4f5c-9f9a-f35d5ff229782022-03-26T16:46:31ZInfrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:55cd0288-01a1-4f5c-9f9a-f35d5ff22978EnglishSymplectic Elements at OxfordWiley2015Ash, PHealy, AVincent, KHidalgo Gonzalez, RWe demonstrate a novel in situ infrared spectroscopic approach for the characterization of hydrogenase during catalytic turnover. E. coli hydrogenase 1 (Hyd-1) is adsorbed on a high surface-area carbon electrode and subjected to the same electrochemical control and efficient supply of substrate as in protein film electrochemistry during spectral acquisition. The spectra reveal that the active site state known as Ni-L, observed in other NiFe hydrogenases only under illumination or at cryogenic temperatures, can be generated reversibly in the dark at ambient temperature under both turnover and non-turnover conditions. The observation that Ni-L is present at all potentials during turnover under H2, suggests that the final steps in the catalytic cycle of H2 oxidation by Hyd-1 involve sequential proton- and electron-transfer via Ni-L. We present a broadly applicable IR spectroscopic technique for addressing electrode-adsorbed redox enzymes under fast catalytic turnover. |
| spellingShingle | Ash, P Healy, A Vincent, K Hidalgo Gonzalez, R Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase. |
| title | Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase. |
| title_full | Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase. |
| title_fullStr | Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase. |
| title_full_unstemmed | Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase. |
| title_short | Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase. |
| title_sort | infrared spectroscopy during electrocatalytic turnover reveals the ni l active site state during h2 oxidation by a nife hydrogenase |
| work_keys_str_mv | AT ashp infraredspectroscopyduringelectrocatalyticturnoverrevealsthenilactivesitestateduringh2oxidationbyanifehydrogenase AT healya infraredspectroscopyduringelectrocatalyticturnoverrevealsthenilactivesitestateduringh2oxidationbyanifehydrogenase AT vincentk infraredspectroscopyduringelectrocatalyticturnoverrevealsthenilactivesitestateduringh2oxidationbyanifehydrogenase AT hidalgogonzalezr infraredspectroscopyduringelectrocatalyticturnoverrevealsthenilactivesitestateduringh2oxidationbyanifehydrogenase |