Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells
The caspase-mediated regulation of many cellular processes, including apoptosis, justifies the substantial interest in understanding all of the biological features of these enzymes. To complement functional assays, it is crucial to identify caspase-activating cells in live tissues. Our work describe...
| Main Authors: | , , , , , |
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| Format: | Journal article |
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Company of Biologists
2018
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| _version_ | 1826273131691507712 |
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| author | Baena-Lopez, L Arthurton, L Bischoff, M Vincent, J Alexandre, C McGregor, R |
| author_facet | Baena-Lopez, L Arthurton, L Bischoff, M Vincent, J Alexandre, C McGregor, R |
| author_sort | Baena-Lopez, L |
| collection | OXFORD |
| description | The caspase-mediated regulation of many cellular processes, including apoptosis, justifies the substantial interest in understanding all of the biological features of these enzymes. To complement functional assays, it is crucial to identify caspase-activating cells in live tissues. Our work describes novel initiator caspase reporters that, for the first time, provide direct information concerning the initial steps of the caspase activation cascade in Drosophila tissues. One of our caspase sensors capitalises on the rapid subcellular localisation change of a fluorescent marker to uncover novel cellular apoptotic events relating to the actin-mediated positioning of the nucleus before cell delamination. The other construct benefits from caspase-induced nuclear translocation of a QF transcription factor. This feature enables the genetic manipulation of caspase-activating cells and reveals the spatiotemporal patterns of initiator caspase activity. Collectively, our sensors offer experimental opportunities not available by using previous reporters and have proven useful to illuminate previously unknown aspects of caspase-dependent processes in apoptotic and non-apoptotic cellular scenarios. |
| first_indexed | 2024-03-06T22:23:31Z |
| format | Journal article |
| id | oxford-uuid:55dbf134-358c-4422-afda-58bc5184cb38 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T22:23:31Z |
| publishDate | 2018 |
| publisher | Company of Biologists |
| record_format | dspace |
| spelling | oxford-uuid:55dbf134-358c-4422-afda-58bc5184cb382022-03-26T16:46:53ZNovel initiator caspase reporters uncover previously unknown features of caspase-activating cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:55dbf134-358c-4422-afda-58bc5184cb38Symplectic Elements at OxfordCompany of Biologists2018Baena-Lopez, LArthurton, LBischoff, MVincent, JAlexandre, CMcGregor, RThe caspase-mediated regulation of many cellular processes, including apoptosis, justifies the substantial interest in understanding all of the biological features of these enzymes. To complement functional assays, it is crucial to identify caspase-activating cells in live tissues. Our work describes novel initiator caspase reporters that, for the first time, provide direct information concerning the initial steps of the caspase activation cascade in Drosophila tissues. One of our caspase sensors capitalises on the rapid subcellular localisation change of a fluorescent marker to uncover novel cellular apoptotic events relating to the actin-mediated positioning of the nucleus before cell delamination. The other construct benefits from caspase-induced nuclear translocation of a QF transcription factor. This feature enables the genetic manipulation of caspase-activating cells and reveals the spatiotemporal patterns of initiator caspase activity. Collectively, our sensors offer experimental opportunities not available by using previous reporters and have proven useful to illuminate previously unknown aspects of caspase-dependent processes in apoptotic and non-apoptotic cellular scenarios. |
| spellingShingle | Baena-Lopez, L Arthurton, L Bischoff, M Vincent, J Alexandre, C McGregor, R Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells |
| title | Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells |
| title_full | Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells |
| title_fullStr | Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells |
| title_full_unstemmed | Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells |
| title_short | Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells |
| title_sort | novel initiator caspase reporters uncover previously unknown features of caspase activating cells |
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