Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats.
Dual-energy X-ray absorptiometry (DXA) is a novel, non-invasive technique for the measurement of gross body composition in small animals. In the present study the absolute accuracy of the Hologic QDR-1000W scanner was assessed by comparison with direct analysis in twelve rats with a range of body fa...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1996
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| _version_ | 1797069392734846976 |
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| author | Jebb, SA Garland, S Jennings, G Elia, M |
| author_facet | Jebb, SA Garland, S Jennings, G Elia, M |
| author_sort | Jebb, SA |
| collection | OXFORD |
| description | Dual-energy X-ray absorptiometry (DXA) is a novel, non-invasive technique for the measurement of gross body composition in small animals. In the present study the absolute accuracy of the Hologic QDR-1000W scanner was assessed by comparison with direct analysis in twelve rats with a range of body fat and bone mineral content (BMC) values. Fat masses measured by DXA and petroleum-ether extraction were significantly different (P < 0.0023). The DXA technique consistently overestimated fat mass by approximately one third of the measured fat content. BMC derived from the measurement of Ca in ash gave a mean of 8.26 (range 1.57-15.71)g. BMC measured by DXA was not significantly different for the group as a whole. However, there was a trend for DXA to overestimate BMC in animals with low BMC and underestimate in those with higher BMC, compared with direct analysis, such that the 95% limits of agreement for the two techniques were +2.73 to -2.58 g. These results suggest that the present small-animal software developed for use with currently available Hologic machines does not give an accurate measure of gross body composition compared with the results from classical direct analysis. |
| first_indexed | 2024-03-06T22:23:46Z |
| format | Journal article |
| id | oxford-uuid:55f3434f-e0e4-4128-8549-5ae3b6ef0730 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:23:46Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:55f3434f-e0e4-4128-8549-5ae3b6ef07302022-03-26T16:47:21ZDual-energy X-ray absorptiometry for the measurement of gross body composition in rats.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:55f3434f-e0e4-4128-8549-5ae3b6ef0730EnglishSymplectic Elements at Oxford1996Jebb, SAGarland, SJennings, GElia, MDual-energy X-ray absorptiometry (DXA) is a novel, non-invasive technique for the measurement of gross body composition in small animals. In the present study the absolute accuracy of the Hologic QDR-1000W scanner was assessed by comparison with direct analysis in twelve rats with a range of body fat and bone mineral content (BMC) values. Fat masses measured by DXA and petroleum-ether extraction were significantly different (P < 0.0023). The DXA technique consistently overestimated fat mass by approximately one third of the measured fat content. BMC derived from the measurement of Ca in ash gave a mean of 8.26 (range 1.57-15.71)g. BMC measured by DXA was not significantly different for the group as a whole. However, there was a trend for DXA to overestimate BMC in animals with low BMC and underestimate in those with higher BMC, compared with direct analysis, such that the 95% limits of agreement for the two techniques were +2.73 to -2.58 g. These results suggest that the present small-animal software developed for use with currently available Hologic machines does not give an accurate measure of gross body composition compared with the results from classical direct analysis. |
| spellingShingle | Jebb, SA Garland, S Jennings, G Elia, M Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats. |
| title | Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats. |
| title_full | Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats. |
| title_fullStr | Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats. |
| title_full_unstemmed | Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats. |
| title_short | Dual-energy X-ray absorptiometry for the measurement of gross body composition in rats. |
| title_sort | dual energy x ray absorptiometry for the measurement of gross body composition in rats |
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