Externalization of annexin I from a folliculo-stellate-like cell line.
Our recent studies on rat pituitary tissue suggest that the annexin I-dependent inhibitory actions of glucocorticoids may not be exerted directly on endocrine cells but indirectly via folliculo-stellate (FS) cells. FS cells contain glucocorticoid receptors and abundant annexin I. We have studied the...
| Autors principals: | , , , , |
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| Format: | Journal article |
| Idioma: | English |
| Publicat: |
2002
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| _version_ | 1826273221761040384 |
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| author | Chapman, L Nishimura, A Buckingham, J Morris, J Christian, H |
| author_facet | Chapman, L Nishimura, A Buckingham, J Morris, J Christian, H |
| author_sort | Chapman, L |
| collection | OXFORD |
| description | Our recent studies on rat pituitary tissue suggest that the annexin I-dependent inhibitory actions of glucocorticoids may not be exerted directly on endocrine cells but indirectly via folliculo-stellate (FS) cells. FS cells contain glucocorticoid receptors and abundant annexin I. We have studied the localization of annexin I in FS cells and the ability of dexamethasone to induce annexin I secretion by an FS (TtT/GF) cell line, using Western blotting and immunofluorescence microscopy. Exposure of TtT/GF cells to dexamethasone (0.1 micro M, 3 h) caused an increase in the amount of annexin I protein in the intracellular compartment and attached to the surface of the cells. In nonpermeabilized cells, immunofluorescence labeling revealed that annexin I immunoreactivity was associated with the cell surface and concentrated in focal patches on the ends of cytoplasmic processes; dexamethasone (0.1 micro M, 3 h) increased both the number and intensity of these foci. Immunogold electron microscopy confirmed in anterior pituitary tissue the presence of immunoreactive-annexin at the surface of FS cell processes contacting endocrine cells. These data support our hypothesis that annexin I is released by FS cells in response to glucocorticoids to mediate glucocorticoid inhibitory actions on pituitary hormone release via a juxtacrine mechanism. |
| first_indexed | 2024-03-06T22:24:54Z |
| format | Journal article |
| id | oxford-uuid:5655d0ae-04df-42ec-bfbd-7b6e3f14ff9f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:24:54Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:5655d0ae-04df-42ec-bfbd-7b6e3f14ff9f2022-03-26T16:49:41ZExternalization of annexin I from a folliculo-stellate-like cell line.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5655d0ae-04df-42ec-bfbd-7b6e3f14ff9fEnglishSymplectic Elements at Oxford2002Chapman, LNishimura, ABuckingham, JMorris, JChristian, HOur recent studies on rat pituitary tissue suggest that the annexin I-dependent inhibitory actions of glucocorticoids may not be exerted directly on endocrine cells but indirectly via folliculo-stellate (FS) cells. FS cells contain glucocorticoid receptors and abundant annexin I. We have studied the localization of annexin I in FS cells and the ability of dexamethasone to induce annexin I secretion by an FS (TtT/GF) cell line, using Western blotting and immunofluorescence microscopy. Exposure of TtT/GF cells to dexamethasone (0.1 micro M, 3 h) caused an increase in the amount of annexin I protein in the intracellular compartment and attached to the surface of the cells. In nonpermeabilized cells, immunofluorescence labeling revealed that annexin I immunoreactivity was associated with the cell surface and concentrated in focal patches on the ends of cytoplasmic processes; dexamethasone (0.1 micro M, 3 h) increased both the number and intensity of these foci. Immunogold electron microscopy confirmed in anterior pituitary tissue the presence of immunoreactive-annexin at the surface of FS cell processes contacting endocrine cells. These data support our hypothesis that annexin I is released by FS cells in response to glucocorticoids to mediate glucocorticoid inhibitory actions on pituitary hormone release via a juxtacrine mechanism. |
| spellingShingle | Chapman, L Nishimura, A Buckingham, J Morris, J Christian, H Externalization of annexin I from a folliculo-stellate-like cell line. |
| title | Externalization of annexin I from a folliculo-stellate-like cell line. |
| title_full | Externalization of annexin I from a folliculo-stellate-like cell line. |
| title_fullStr | Externalization of annexin I from a folliculo-stellate-like cell line. |
| title_full_unstemmed | Externalization of annexin I from a folliculo-stellate-like cell line. |
| title_short | Externalization of annexin I from a folliculo-stellate-like cell line. |
| title_sort | externalization of annexin i from a folliculo stellate like cell line |
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