Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans.
A study of the final stages of the biosynthesis of the penicillins in Penicillium chrysogenum has revealed two types of enzyme. One hydrolyses phenoxymethyl penicillin to 6-aminopenicillanic acid (6-APA). The other, also obtained from Aspergillus nidulans, transfers a phenylacetyl group from phenyla...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1990
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| _version_ | 1797069595603894272 |
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| author | Whiteman, P Abraham, E Baldwin, J Fleming, MD Schofield, C Sutherland, J Willis, A |
| author_facet | Whiteman, P Abraham, E Baldwin, J Fleming, MD Schofield, C Sutherland, J Willis, A |
| author_sort | Whiteman, P |
| collection | OXFORD |
| description | A study of the final stages of the biosynthesis of the penicillins in Penicillium chrysogenum has revealed two types of enzyme. One hydrolyses phenoxymethyl penicillin to 6-aminopenicillanic acid (6-APA). The other, also obtained from Aspergillus nidulans, transfers a phenylacetyl group from phenylacetyl CoA to 6-APA. The acyltransferase, purified to apparent homogeneity, had a molecular mass of 40 kDa. It also catalyses the conversion of isopenicillin N (IPN) to benzylpenicillin (Pen G) and hydrolyses IPN to 6-APA. In the presence of SDS it dissociates, with loss of activity, into fragments of ca 30 and 10.5 kDa, but activity is regained when these fragments recombine in the absence of SDS. |
| first_indexed | 2024-03-06T22:26:47Z |
| format | Journal article |
| id | oxford-uuid:56edcec1-fdeb-427f-95f1-4df34b65cfc9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:26:47Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:56edcec1-fdeb-427f-95f1-4df34b65cfc92022-03-26T16:53:25ZAcyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:56edcec1-fdeb-427f-95f1-4df34b65cfc9EnglishSymplectic Elements at Oxford1990Whiteman, PAbraham, EBaldwin, JFleming, MDSchofield, CSutherland, JWillis, AA study of the final stages of the biosynthesis of the penicillins in Penicillium chrysogenum has revealed two types of enzyme. One hydrolyses phenoxymethyl penicillin to 6-aminopenicillanic acid (6-APA). The other, also obtained from Aspergillus nidulans, transfers a phenylacetyl group from phenylacetyl CoA to 6-APA. The acyltransferase, purified to apparent homogeneity, had a molecular mass of 40 kDa. It also catalyses the conversion of isopenicillin N (IPN) to benzylpenicillin (Pen G) and hydrolyses IPN to 6-APA. In the presence of SDS it dissociates, with loss of activity, into fragments of ca 30 and 10.5 kDa, but activity is regained when these fragments recombine in the absence of SDS. |
| spellingShingle | Whiteman, P Abraham, E Baldwin, J Fleming, MD Schofield, C Sutherland, J Willis, A Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans. |
| title | Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans. |
| title_full | Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans. |
| title_fullStr | Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans. |
| title_full_unstemmed | Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans. |
| title_short | Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans. |
| title_sort | acyl coenzyme a 6 aminopenicillanic acid acyltransferase from penicillium chrysogenum and aspergillus nidulans |
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