CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3.

CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3.

The prokaryotic CRISPR/Cas immune system is based on genomic loci that contain incorporated sequence tags from viruses and plasmids. Using small guide RNA molecules, these sequences act as a memory to reject returning invaders. Both the Cascade ribonucleoprotein complex and the Cas3 nuclease/helicas...

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Bibliographic Details
Main Authors: Westra, E, van Erp, P, Künne, T, Wong, S, Staals, R, Seegers, C, Bollen, S, Jore, M, Semenova, E, Severinov, K, de Vos, WM, Dame, RT, de Vries, R, Brouns, S, van der Oost, J
Format: Journal article
Language:English
Published: 2012

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