A novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein.
Here we report that the OX-TES-1 SEREX antigen, which showed immunological reactivity with serum from four out of 10 diffuse large B-cell lymphoma (DLBCL) patients, is encoded by a novel gene, PAS domain containing 1 (PASD1). PASD1_v1 cDNA encodes a 639 amino-acid (aa) protein product, while an alte...
| Hlavní autoři: | , , , , |
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| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
2004
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| _version_ | 1826273456098902016 |
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| author | Liggins, A Brown, P Asker, K Pulford, K Banham, A |
| author_facet | Liggins, A Brown, P Asker, K Pulford, K Banham, A |
| author_sort | Liggins, A |
| collection | OXFORD |
| description | Here we report that the OX-TES-1 SEREX antigen, which showed immunological reactivity with serum from four out of 10 diffuse large B-cell lymphoma (DLBCL) patients, is encoded by a novel gene, PAS domain containing 1 (PASD1). PASD1_v1 cDNA encodes a 639 amino-acid (aa) protein product, while an alternatively spliced variant (PASD1_v2), lacking intron 14, encodes a 773 aa protein, the first 638 aa of which are common to both proteins. The PASD1-predicted protein contains a PAS domain that, together with a putative leucine zipper and nuclear localisation signal, suggests it encodes a transcription factor. The expression of PASD1_v1 mRNA was confirmed by RT-PCR in seven DLBCL-derived cell lines, while PASD1_v2 mRNA appears to be preferentially expressed in cell lines derived from non-germinal centre DLBCL. Immunophenotyping studies of de novo DLBCL patients' tumours with antibodies to CD10, BCL-6 and MUM1 indicated that two patients mounting an immune response to PASD1 were of a poor prognosis non-germinal centre subtype. Expression of PASD1 mRNA was restricted to normal testis, while frequent expression was observed in solid tumours (25 out of 68), thus fulfilling the criteria for a novel cancer testis antigen. PASD1 has potential for lymphoma vaccine development that may also be widely applicable to other tumour types. |
| first_indexed | 2024-03-06T22:28:28Z |
| format | Journal article |
| id | oxford-uuid:57777aff-1c3e-4fe8-a5c6-1b5b8a9ac7b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:28:28Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:57777aff-1c3e-4fe8-a5c6-1b5b8a9ac7b42022-03-26T16:56:55ZA novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:57777aff-1c3e-4fe8-a5c6-1b5b8a9ac7b4EnglishSymplectic Elements at Oxford2004Liggins, ABrown, PAsker, KPulford, KBanham, AHere we report that the OX-TES-1 SEREX antigen, which showed immunological reactivity with serum from four out of 10 diffuse large B-cell lymphoma (DLBCL) patients, is encoded by a novel gene, PAS domain containing 1 (PASD1). PASD1_v1 cDNA encodes a 639 amino-acid (aa) protein product, while an alternatively spliced variant (PASD1_v2), lacking intron 14, encodes a 773 aa protein, the first 638 aa of which are common to both proteins. The PASD1-predicted protein contains a PAS domain that, together with a putative leucine zipper and nuclear localisation signal, suggests it encodes a transcription factor. The expression of PASD1_v1 mRNA was confirmed by RT-PCR in seven DLBCL-derived cell lines, while PASD1_v2 mRNA appears to be preferentially expressed in cell lines derived from non-germinal centre DLBCL. Immunophenotyping studies of de novo DLBCL patients' tumours with antibodies to CD10, BCL-6 and MUM1 indicated that two patients mounting an immune response to PASD1 were of a poor prognosis non-germinal centre subtype. Expression of PASD1 mRNA was restricted to normal testis, while frequent expression was observed in solid tumours (25 out of 68), thus fulfilling the criteria for a novel cancer testis antigen. PASD1 has potential for lymphoma vaccine development that may also be widely applicable to other tumour types. |
| spellingShingle | Liggins, A Brown, P Asker, K Pulford, K Banham, A A novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein. |
| title | A novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein. |
| title_full | A novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein. |
| title_fullStr | A novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein. |
| title_full_unstemmed | A novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein. |
| title_short | A novel diffuse large B-cell lymphoma-associated cancer testis antigen encoding a PAS domain protein. |
| title_sort | novel diffuse large b cell lymphoma associated cancer testis antigen encoding a pas domain protein |
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