Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype.
Tumor-associated macrophages (TAM) may have tumor-promoting activity, but it is not clear how their phenotype is achieved. In this study, we demonstrate that ovarian cancer cells switch cocultured macrophages to a phenotype similar to that found in ovarian tumors. Tumor cells caused dynamic changes...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2006
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| _version_ | 1797069715538968576 |
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| author | Hagemann, T Wilson, J Burke, F Kulbe, H Li, N Plüddemann, A Charles, K Gordon, S Balkwill, F |
| author_facet | Hagemann, T Wilson, J Burke, F Kulbe, H Li, N Plüddemann, A Charles, K Gordon, S Balkwill, F |
| author_sort | Hagemann, T |
| collection | OXFORD |
| description | Tumor-associated macrophages (TAM) may have tumor-promoting activity, but it is not clear how their phenotype is achieved. In this study, we demonstrate that ovarian cancer cells switch cocultured macrophages to a phenotype similar to that found in ovarian tumors. Tumor cells caused dynamic changes in macrophage cytokine, chemokine, and matrix metalloprotease mRNA, and protein-inducing mediators that are found in human cancer. Macrophage mannose, mannose receptor, and scavenger receptors (SR-As) were also up-regulated by coculture, but not by conditioned medium. To further validate the model, we studied SR-A regulation on TAM in vitro and in vivo. Coculture of murine macrophages from mice deficient in TNF-alpha or its receptors revealed that TNF-alpha was key to SR-A induction via its p75 receptor. SR-A expression was also reduced in TAM from ovarian cancers treated with anti-TNF-alpha Abs or grown in TNF-alpha(-/-) mice. Chemical communication between tumor cells and macrophages may be important in regulating the cancer cytokine microenvironment. |
| first_indexed | 2024-03-06T22:28:33Z |
| format | Journal article |
| id | oxford-uuid:577f2130-11cc-4ad9-94e8-653b6508b7c4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:28:33Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:577f2130-11cc-4ad9-94e8-653b6508b7c42022-03-26T16:57:08ZOvarian cancer cells polarize macrophages toward a tumor-associated phenotype.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:577f2130-11cc-4ad9-94e8-653b6508b7c4EnglishSymplectic Elements at Oxford2006Hagemann, TWilson, JBurke, FKulbe, HLi, NPlüddemann, ACharles, KGordon, SBalkwill, FTumor-associated macrophages (TAM) may have tumor-promoting activity, but it is not clear how their phenotype is achieved. In this study, we demonstrate that ovarian cancer cells switch cocultured macrophages to a phenotype similar to that found in ovarian tumors. Tumor cells caused dynamic changes in macrophage cytokine, chemokine, and matrix metalloprotease mRNA, and protein-inducing mediators that are found in human cancer. Macrophage mannose, mannose receptor, and scavenger receptors (SR-As) were also up-regulated by coculture, but not by conditioned medium. To further validate the model, we studied SR-A regulation on TAM in vitro and in vivo. Coculture of murine macrophages from mice deficient in TNF-alpha or its receptors revealed that TNF-alpha was key to SR-A induction via its p75 receptor. SR-A expression was also reduced in TAM from ovarian cancers treated with anti-TNF-alpha Abs or grown in TNF-alpha(-/-) mice. Chemical communication between tumor cells and macrophages may be important in regulating the cancer cytokine microenvironment. |
| spellingShingle | Hagemann, T Wilson, J Burke, F Kulbe, H Li, N Plüddemann, A Charles, K Gordon, S Balkwill, F Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype. |
| title | Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype. |
| title_full | Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype. |
| title_fullStr | Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype. |
| title_full_unstemmed | Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype. |
| title_short | Ovarian cancer cells polarize macrophages toward a tumor-associated phenotype. |
| title_sort | ovarian cancer cells polarize macrophages toward a tumor associated phenotype |
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