Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells.
Dendritic cells (DC) are professional antigen-presenting cells that are able to induce primary T cell responses. Therefore, several strategies employ peptide-pulsed DC in tumor immunotherapy. For efficient antigen presentation and induction of an immune response by DC the number and stability of MHC...
| Asıl Yazarlar: | , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
2000
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| _version_ | 1826273541965742080 |
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| author | Kukutsch, N Rossner, S Austyn, J Schuler, G Lutz, M |
| author_facet | Kukutsch, N Rossner, S Austyn, J Schuler, G Lutz, M |
| author_sort | Kukutsch, N |
| collection | OXFORD |
| description | Dendritic cells (DC) are professional antigen-presenting cells that are able to induce primary T cell responses. Therefore, several strategies employ peptide-pulsed DC in tumor immunotherapy. For efficient antigen presentation and induction of an immune response by DC the number and stability of MHC I-peptide complexes is crucial. We studied this issue by using the antibody 25-D1.16 that specifically detects OVA peptide SIINFEKL in conjunction with H-2 Kb molecules, and determined its kinetics on mature and immature bone marrow-derived murine DC. Optimal peptide loading was reached after 8-16 h at 50 microM peptide pulse, and was comparable in serum-free versus serum-containing medium. Stimulation of DC with LPS or Poly I:C, and to a lesser extent TNF-alpha, upregulated the total number of surface MHC I molecules and thus improved peptide loading. Pulse-chase experiments revealed a constant half-life of peptide/Kb complexes independent of preceding DC stimulation or their maturation stage. The duration of peptide/Kb complex expression on mature DC, however, could be extended from 24 h to 72 h when the cultures were pretreated with LPS or Poly I:C, but not TNF-alpha. These data might have important implications for the clinical application of peptide-pulsed DC in tumor immunotherapy. |
| first_indexed | 2024-03-06T22:29:46Z |
| format | Journal article |
| id | oxford-uuid:57e4f154-f1eb-4d48-bc86-1dcf18c43b72 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:29:46Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:57e4f154-f1eb-4d48-bc86-1dcf18c43b722022-03-26T16:59:32ZFormation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:57e4f154-f1eb-4d48-bc86-1dcf18c43b72EnglishSymplectic Elements at Oxford2000Kukutsch, NRossner, SAustyn, JSchuler, GLutz, MDendritic cells (DC) are professional antigen-presenting cells that are able to induce primary T cell responses. Therefore, several strategies employ peptide-pulsed DC in tumor immunotherapy. For efficient antigen presentation and induction of an immune response by DC the number and stability of MHC I-peptide complexes is crucial. We studied this issue by using the antibody 25-D1.16 that specifically detects OVA peptide SIINFEKL in conjunction with H-2 Kb molecules, and determined its kinetics on mature and immature bone marrow-derived murine DC. Optimal peptide loading was reached after 8-16 h at 50 microM peptide pulse, and was comparable in serum-free versus serum-containing medium. Stimulation of DC with LPS or Poly I:C, and to a lesser extent TNF-alpha, upregulated the total number of surface MHC I molecules and thus improved peptide loading. Pulse-chase experiments revealed a constant half-life of peptide/Kb complexes independent of preceding DC stimulation or their maturation stage. The duration of peptide/Kb complex expression on mature DC, however, could be extended from 24 h to 72 h when the cultures were pretreated with LPS or Poly I:C, but not TNF-alpha. These data might have important implications for the clinical application of peptide-pulsed DC in tumor immunotherapy. |
| spellingShingle | Kukutsch, N Rossner, S Austyn, J Schuler, G Lutz, M Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells. |
| title | Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells. |
| title_full | Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells. |
| title_fullStr | Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells. |
| title_full_unstemmed | Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells. |
| title_short | Formation and kinetics of MHC class I-ovalbumin peptide complexes on immature and mature murine dendritic cells. |
| title_sort | formation and kinetics of mhc class i ovalbumin peptide complexes on immature and mature murine dendritic cells |
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