Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density
Regulation of plasma membrane curvature and composition governs essential cellular processes. The material property of bending rigidity describes the energetic cost of membrane deformations and depends on the plasma membrane molecular composition. Because of compositional fluctuations and active pro...
| Main Authors: | , , , , |
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| 格式: | Journal article |
| 語言: | English |
| 出版: |
Nature Research
2019
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| _version_ | 1826273561467158528 |
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| author | Steinkühler, J Sezgin, E Urbančič, I Eggeling, C Dimova, R |
| author_facet | Steinkühler, J Sezgin, E Urbančič, I Eggeling, C Dimova, R |
| author_sort | Steinkühler, J |
| collection | OXFORD |
| description | Regulation of plasma membrane curvature and composition governs essential cellular processes. The material property of bending rigidity describes the energetic cost of membrane deformations and depends on the plasma membrane molecular composition. Because of compositional fluctuations and active processes, it is challenging to measure it in intact cells. Here, we study the plasma membrane using giant plasma membrane vesicles (GPMVs), which largely preserve the plasma membrane lipidome and proteome. We show that the bending rigidity of plasma membranes under varied conditions is correlated to readout from environment-sensitive dyes, which are indicative of membrane order and microviscosity. This correlation holds across different cell lines, upon cholesterol depletion or enrichment of the plasma membrane, and variations in cell density. Thus, polarity- and viscosity-sensitive probes represent a promising indicator of membrane mechanical properties. Additionally, our results allow for identifying synthetic membranes with a few well defined lipids as optimal plasma membrane mimetics. |
| first_indexed | 2024-03-06T22:30:04Z |
| format | Journal article |
| id | oxford-uuid:57fc70a9-46f9-4209-a95c-d42e88e8ef51 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:30:04Z |
| publishDate | 2019 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:57fc70a9-46f9-4209-a95c-d42e88e8ef512022-03-26T17:00:11ZMechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell densityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:57fc70a9-46f9-4209-a95c-d42e88e8ef51EnglishSymplectic Elements at OxfordNature Research2019Steinkühler, JSezgin, EUrbančič, IEggeling, CDimova, RRegulation of plasma membrane curvature and composition governs essential cellular processes. The material property of bending rigidity describes the energetic cost of membrane deformations and depends on the plasma membrane molecular composition. Because of compositional fluctuations and active processes, it is challenging to measure it in intact cells. Here, we study the plasma membrane using giant plasma membrane vesicles (GPMVs), which largely preserve the plasma membrane lipidome and proteome. We show that the bending rigidity of plasma membranes under varied conditions is correlated to readout from environment-sensitive dyes, which are indicative of membrane order and microviscosity. This correlation holds across different cell lines, upon cholesterol depletion or enrichment of the plasma membrane, and variations in cell density. Thus, polarity- and viscosity-sensitive probes represent a promising indicator of membrane mechanical properties. Additionally, our results allow for identifying synthetic membranes with a few well defined lipids as optimal plasma membrane mimetics. |
| spellingShingle | Steinkühler, J Sezgin, E Urbančič, I Eggeling, C Dimova, R Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density |
| title | Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density |
| title_full | Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density |
| title_fullStr | Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density |
| title_full_unstemmed | Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density |
| title_short | Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density |
| title_sort | mechanical properties of plasma membrane vesicles correlate with lipid order viscosity and cell density |
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