The interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infection
The outcome of HBeAg-negative chronic hepatitis B virus (HBV) patients who may remain in the inactive carrier state (IC) or progress to HBeAg-negative chronic hepatitis B may be affected by the host genetic profile. Genetic polymorphisms within not only the promoter but also the coding sequence of t...
| প্রধান লেখক: | , , , , , , , |
|---|---|
| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
Wiley
2015
|
| _version_ | 1826273573339136000 |
|---|---|
| author | Karamitros, T Papatheodoridis, G Dimopoulou, E Papageorgiou, M Paraskevis, D Magiorkinis, G Sypsa, V Hatzakis, A |
| author_facet | Karamitros, T Papatheodoridis, G Dimopoulou, E Papageorgiou, M Paraskevis, D Magiorkinis, G Sypsa, V Hatzakis, A |
| author_sort | Karamitros, T |
| collection | OXFORD |
| description | The outcome of HBeAg-negative chronic hepatitis B virus (HBV) patients who may remain in the inactive carrier state (IC) or progress to HBeAg-negative chronic hepatitis B may be affected by the host genetic profile. Genetic polymorphisms within not only the promoter but also the coding sequence of the interferon receptor 1 (INFAR1) gene have been associated with susceptibility to chronic HBV infection, but their role on the outcomes of HBeAg-negative patients has not been evaluated. We examined the association of INFAR1 promoter polymorphisms with the phase of chronic HBV infection in a demographically characterized Caucasian cohort of 183 consecutive HBeAg-negative chronic HBV patients.Using a combination of conventional and allele-specific polymerase chain reactions, bidirectional sequencing and DNA-fragment analysis, we performed typing of three Single Nucleotide Polymorphisms (SNPs -568G/C, -408C/T, -3C/T) and one Variable Number Tandem Repeat [VNTR -77(GT)n] within the INFR1 promoter sequence.The genetic polymorphisms examined were found to be associated with the phase of HBeAg-negative chronic HBV patients. Using a multiple logistic regression model adjusting for age, gender and origin of the individuals, we found that patients with linked genotypes -408CT_-3CT were more likely to be ICs (OR = 2.42 vs. CC, P = 0.036). Also, given the partial linkage between SNP -568G/C and VNTR -77(GT)n, we found that linked genotypes -77(GT)n ≤ 8/≤8_-568GC and -77(GT)n ≤ 8/≤8_-568CC were detected more frequently among ICs (OR = 11.69, P = 0.005 and OR = 7.56, P = 0.001 vs. -77(GT)n >8/>8_-568GG respectively).These findings suggest that these genetic variations represent important factors associated with the clinical phase of HBeAg-negative chronic HBV infection. |
| first_indexed | 2024-03-06T22:30:15Z |
| format | Journal article |
| id | oxford-uuid:580a7cbe-cc29-4be4-bccd-c66e475b46d9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:30:15Z |
| publishDate | 2015 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:580a7cbe-cc29-4be4-bccd-c66e475b46d92022-03-26T17:00:32ZThe interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infectionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:580a7cbe-cc29-4be4-bccd-c66e475b46d9EnglishSymplectic Elements at OxfordWiley2015Karamitros, TPapatheodoridis, GDimopoulou, EPapageorgiou, MParaskevis, DMagiorkinis, GSypsa, VHatzakis, AThe outcome of HBeAg-negative chronic hepatitis B virus (HBV) patients who may remain in the inactive carrier state (IC) or progress to HBeAg-negative chronic hepatitis B may be affected by the host genetic profile. Genetic polymorphisms within not only the promoter but also the coding sequence of the interferon receptor 1 (INFAR1) gene have been associated with susceptibility to chronic HBV infection, but their role on the outcomes of HBeAg-negative patients has not been evaluated. We examined the association of INFAR1 promoter polymorphisms with the phase of chronic HBV infection in a demographically characterized Caucasian cohort of 183 consecutive HBeAg-negative chronic HBV patients.Using a combination of conventional and allele-specific polymerase chain reactions, bidirectional sequencing and DNA-fragment analysis, we performed typing of three Single Nucleotide Polymorphisms (SNPs -568G/C, -408C/T, -3C/T) and one Variable Number Tandem Repeat [VNTR -77(GT)n] within the INFR1 promoter sequence.The genetic polymorphisms examined were found to be associated with the phase of HBeAg-negative chronic HBV patients. Using a multiple logistic regression model adjusting for age, gender and origin of the individuals, we found that patients with linked genotypes -408CT_-3CT were more likely to be ICs (OR = 2.42 vs. CC, P = 0.036). Also, given the partial linkage between SNP -568G/C and VNTR -77(GT)n, we found that linked genotypes -77(GT)n ≤ 8/≤8_-568GC and -77(GT)n ≤ 8/≤8_-568CC were detected more frequently among ICs (OR = 11.69, P = 0.005 and OR = 7.56, P = 0.001 vs. -77(GT)n >8/>8_-568GG respectively).These findings suggest that these genetic variations represent important factors associated with the clinical phase of HBeAg-negative chronic HBV infection. |
| spellingShingle | Karamitros, T Papatheodoridis, G Dimopoulou, E Papageorgiou, M Paraskevis, D Magiorkinis, G Sypsa, V Hatzakis, A The interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infection |
| title | The interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infection |
| title_full | The interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infection |
| title_fullStr | The interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infection |
| title_full_unstemmed | The interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infection |
| title_short | The interferon receptor-1 promoter polymorphisms affect the outcome of Caucasians with HBeAg-negative chronic HBV infection |
| title_sort | interferon receptor 1 promoter polymorphisms affect the outcome of caucasians with hbeag negative chronic hbv infection |
| work_keys_str_mv | AT karamitrost theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT papatheodoridisg theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT dimopouloue theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT papageorgioum theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT paraskevisd theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT magiorkinisg theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT sypsav theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT hatzakisa theinterferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT karamitrost interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT papatheodoridisg interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT dimopouloue interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT papageorgioum interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT paraskevisd interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT magiorkinisg interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT sypsav interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection AT hatzakisa interferonreceptor1promoterpolymorphismsaffecttheoutcomeofcaucasianswithhbeagnegativechronichbvinfection |