A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

Background: The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate mem...

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Bibliographic Details
Main Authors: Long, A, O'Brien, C, Malhotra, K, Schwall, C, Albert, A, Watts, A, Alder, N
Format: Journal article
Language:English
Published: BioMed Central 2013