The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast
Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potentia...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
eLife Sciences Publications
2019
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| _version_ | 1826273666972778496 |
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| author | Wong, I Neo, J Oehler, J Schafhauser, S Osman, F Carr, S Whitby, M |
| author_facet | Wong, I Neo, J Oehler, J Schafhauser, S Osman, F Carr, S Whitby, M |
| author_sort | Wong, I |
| collection | OXFORD |
| description | Previously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro. |
| first_indexed | 2024-03-06T22:31:42Z |
| format | Journal article |
| id | oxford-uuid:587f4c15-20d2-46c7-8136-bf47db44341f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:31:42Z |
| publishDate | 2019 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:587f4c15-20d2-46c7-8136-bf47db44341f2022-03-26T17:03:47ZThe Fml1-MHF complex suppresses inter-fork strand annealing in fission yeastJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:587f4c15-20d2-46c7-8136-bf47db44341fEnglishSymplectic Elements at OxfordeLife Sciences Publications2019Wong, INeo, JOehler, JSchafhauser, SOsman, FCarr, SWhitby, MPreviously we reported that a process called inter-fork strand annealing (IFSA) causes genomic deletions during the termination of DNA replication when an active replication fork converges on a collapsed fork (Morrow et al., 2017). We also identified the FANCM-related DNA helicase Fml1 as a potential suppressor of IFSA. Here, we confirm that Fml1 does indeed suppress IFSA, and show that this function depends on its catalytic activity and ability to interact with Mhf1-Mhf2 via its C-terminal domain. Finally, a plausible mechanism of IFSA suppression is demonstrated by the finding that Fml1 can catalyse regressed fork restoration in vitro. |
| spellingShingle | Wong, I Neo, J Oehler, J Schafhauser, S Osman, F Carr, S Whitby, M The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast |
| title | The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast |
| title_full | The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast |
| title_fullStr | The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast |
| title_full_unstemmed | The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast |
| title_short | The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast |
| title_sort | fml1 mhf complex suppresses inter fork strand annealing in fission yeast |
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