Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater.
AIMS: To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater. METHODS AND RESULTS: One-litre microcosms of groundwater were spiked with 100 micromol 1(-1) of TCE and amended w...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1826273747928088576 |
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| author | Brown, JR Thompson, I Paton, G Singer, A |
| author_facet | Brown, JR Thompson, I Paton, G Singer, A |
| author_sort | Brown, JR |
| collection | OXFORD |
| description | AIMS: To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater. METHODS AND RESULTS: One-litre microcosms of groundwater were spiked with 100 micromol 1(-1) of TCE and amended weekly for 16 weeks with 20 microl 1(-1) of the following plant monoterpenes: linalool, pulegone, R-(+) carvone, S-(-) carvone, farnesol, cumene. Yeast extract-amended and unamended control treatments were also prepared. The addition of R-carvone and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88% of the TCE, significantly more than the unamendment control (61%). The aforementioned group of terpenes also significantly (P < 0.05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone), and the yeast extract treatment which biotransformed 74-75% of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls. CONCLUSIONS: TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids. SIGNIFICANCE AND IMPACT OF THE STUDY: Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation. |
| first_indexed | 2024-03-06T22:32:56Z |
| format | Journal article |
| id | oxford-uuid:58de89db-7dd4-42ea-a798-4581c6b0fdef |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:32:56Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:58de89db-7dd4-42ea-a798-4581c6b0fdef2022-03-26T17:06:21ZEnhanced biotransformation of TCE using plant terpenoids in contaminated groundwater.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:58de89db-7dd4-42ea-a798-4581c6b0fdefEnglishSymplectic Elements at Oxford2009Brown, JRThompson, IPaton, GSinger, A AIMS: To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous microbial community originating from a plume of TCE-contaminated groundwater. METHODS AND RESULTS: One-litre microcosms of groundwater were spiked with 100 micromol 1(-1) of TCE and amended weekly for 16 weeks with 20 microl 1(-1) of the following plant monoterpenes: linalool, pulegone, R-(+) carvone, S-(-) carvone, farnesol, cumene. Yeast extract-amended and unamended control treatments were also prepared. The addition of R-carvone and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88% of the TCE, significantly more than the unamendment control (61%). The aforementioned group of terpenes also significantly (P < 0.05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone), and the yeast extract treatment which biotransformed 74-75% of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls. CONCLUSIONS: TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids. SIGNIFICANCE AND IMPACT OF THE STUDY: Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation. |
| spellingShingle | Brown, JR Thompson, I Paton, G Singer, A Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. |
| title | Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. |
| title_full | Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. |
| title_fullStr | Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. |
| title_full_unstemmed | Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. |
| title_short | Enhanced biotransformation of TCE using plant terpenoids in contaminated groundwater. |
| title_sort | enhanced biotransformation of tce using plant terpenoids in contaminated groundwater |
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