Multistep binding of transition metals to the H-N-H endonuclease toxin colicin E9.

Multistep binding of transition metals to the H-N-H endonuclease toxin colicin E9.

We report the first stopped-flow fluorescence analysis of transition metal binding (Co(2+), Ni(2+), Cu(2+), and Zn(2+)) to the H-N-H endonuclease motif within colicin E9 (the E9 DNase). The H-N-H consensus forms the active site core of a number of endonuclease groups but is also structurally homolog...

書誌詳細
主要な著者:	Keeble, A, Hemmings, A, James, R, Moore, G, Kleanthous, K
フォーマット:	Journal article
言語:	English
出版事項:	2002

類似資料

The kinetic basis for dual recognition in colicin endonuclease-immunity protein complexes.
著者:: Keeble, A, 等
出版事項: (2005)

Destabilization of the colicin E9 Endonuclease domain by interaction with negatively charged phospholipids: implications for colicin translocation into bacteria.
著者:: Mosbahi, K, 等
出版事項: (2004)

Distinct conformational stability and functional activity of four highly homologous endonuclease colicins.
著者:: van den Bremer, E, 等
出版事項: (2004)

Experimental and computational analyses of the energetic basis for dual recognition of immunity proteins by colicin endonucleases.
著者:: Keeble, A, 等
出版事項: (2008)

Structure-based analysis of the metal-dependent mechanism of H-N-H endonucleases.
著者:: Maté, M, 等
出版事項: (2004)

Calorimetric dissection of colicin DNase--immunity protein complex specificity.
著者:: Keeble, A, 等
出版事項: (2006)

Characterisation of a mobile protein-binding epitope in the translocation domain of colicin E9.
著者:: Macdonald, C, 等
出版事項: (2004)

Clusters in an intrinsically disordered protein create a protein-binding site: the TolB-binding region of colicin E9.
著者:: Tozawa, K, 等
出版事項: (2005)

Thermodynamic consequences of bipartite immunity protein binding to the ribosomal ribonuclease colicin E3.
著者:: Walker, D, 等
出版事項: (2003)

Thermodynamic dissection of colicin interactions.
著者:: Housden, N, 等
出版事項: (2011)

Flexibility in the receptor-binding domain of the enzymatic colicin E9 is required for toxicity against Escherichia coli cells.
著者:: Penfold, C, 等
出版事項: (2004)

Cell entry mechanism of enzymatic bacterial colicins: porin recruitment and the thermodynamics of receptor binding.
著者:: Housden, N, 等
出版事項: (2005)

Colicin biology.
著者:: Cascales, E, 等
出版事項: (2007)

Nuclease colicins and their immunity proteins.
著者:: Papadakos, G, 等
出版事項: (2012)

Metal induced selectivity in phosphate ion binding in E9 DNase.
著者:: van den Bremer, E, 等
出版事項: (2005)

Colicin translocation across the Escherichia coli outer membrane.
著者:: Housden, N, 等
出版事項: (2012)

Bifurcated binding of the OmpF receptor underpins import of the bacteriocin colicin N into Escherichia coli
著者:: Jansen, KB, 等
出版事項: (2020)

Swimming against the tide: progress and challenges in our understanding of colicin translocation.
著者:: Kleanthous, K
出版事項: (2010)

Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9.
著者:: Loftus, SR, 等
出版事項: (2006)

OmpF enhances the ability of BtuB to protect susceptible Escherichia coli cells from colicin E9 cytotoxicity.
著者:: Law, C, 等
出版事項: (2003)

Kinetic basis for the competitive recruitment of TolB by the intrinsically disordered translocation domain of colicin E9.
著者:: Papadakos, G, 等
出版事項: (2012)

Kinetic basis for the competitive recruitment of TolB by the intrinsically disordered translocation domain of colicin E9
著者:: Papadakos, G, 等
出版事項: (2012)

Rapid detection of colicin E9-induced DNA damage using Escherichia coli cells carrying SOS promoter-lux fusions.
著者:: Vankemmelbeke, M, 等
出版事項: (2005)

Sequential and Multistep Substrate Interrogation Provides the Scaffold for Specificity in Human Flap Endonuclease 1
著者:: Mohamed A. Sobhy, 等
出版事項: (2013-06-01)

The role of electrostatics in colicin nuclease domain translocation into bacterial cells.
著者:: Walker, D, 等
出版事項: (2007)

Global structural rearrangement of the cell penetrating ribonuclease colicin E3 on interaction with phospholipid membranes.
著者:: Mosbahi, K, 等
出版事項: (2006)

Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation.
著者:: Vankemmelbeke, M, 等
出版事項: (2009)

O-antigen dependent colicin insensitivity of uropathogenic Escherichia coli
著者:: Sharp, C, 等
出版事項: (2018)

Directional porin binding of intrinsically disordered protein sequences promotes colicin epitope display in the bacterial periplasm
著者:: Housden, NG, 等
出版事項: (2018)

Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins.
著者:: Bonsor, D, 等
出版事項: (2009)

Ligand-induced changes in the conformational dynamics of a bacterial cytotoxic endonuclease.
著者:: van den Bremer, E, 等
出版事項: (2004)

Identification of the catalytic motif of the microbial ribosome inactivating cytotoxin colicin E3.
著者:: Walker, D, 等
出版事項: (2004)

Colicins exploit native disorder to gain cell entry: a hitchhiker's guide to translocation.
著者:: Bonsor, D, 等
出版事項: (2008)

Colicins exploit native disorder to gain cell entry: A hitchhiker's guide to translocation
著者:: Bonsor, D, 等
出版事項: (2008)

Structural analysis of specific metal chelating inhibitor binding to the endonuclease domain of influenza pH1N1 (2009) polymerase.
著者:: Eva Kowalinski, 等
出版事項: (2012-01-01)

Structure of the ultra-high-affinity colicin E2 DNase--Im2 complex.
著者:: Wojdyla, J, 等
出版事項: (2012)

Structure of the ultra-high-affinity colicin E2 DNase-Im2 complex
著者:: Wojdyla, J, 等
出版事項: (2012)

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
著者:: Wu, Xuebing, 等
出版事項: (2015)

Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3.
著者:: Ng, C, 等
出版事項: (2010)

Colicin-mediated transport of DNA through the iron transporter FepA
著者:: Cohen-Khait, R, 等
出版事項: (2021)