Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase
Successful bacterial circular chromosome segregation requires that any dimeric chromosomes, which arise by crossing over during homologous recombination, are converted to monomers. Resolution of dimers to monomers requires the action of the XerCD site-specific recombinase at dif in the chromosome re...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Blackwell Publishing
2006
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| Subjects: |
| _version_ | 1826273781139636224 |
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| author | Yates, J Zhekov, I Baker, R Eklund, B Sherratt, D Arciszewska, L |
| author_facet | Yates, J Zhekov, I Baker, R Eklund, B Sherratt, D Arciszewska, L |
| author_sort | Yates, J |
| collection | OXFORD |
| description | Successful bacterial circular chromosome segregation requires that any dimeric chromosomes, which arise by crossing over during homologous recombination, are converted to monomers. Resolution of dimers to monomers requires the action of the XerCD site-specific recombinase at dif in the chromosome replication terminus region. This reaction requires the DNA translocase, FtsKc, which activates dimer resolution by catalysing an ATP hydrolysis-dependent switch in the catalytic state of the nucleoprotein recombination complex. We show that a 62-amino-acid fragment of FtsKc interacts directly with the XerD C-terminus in order to stimulate the cleavage by XerD of BSN, a dif-DNA suicide substrate containing a nick in the 'bottom' strand. The resulting recombinase-DNA covalent complex can undergo strand exchange with intact duplex dif in the absence of ATP. Ftskc-mediated stimulation of BSN cleavage by XerD requires synaptic complex formation. Mutational impairment of the XerD-FtsKc interaction leads to reduction in the in vitro stimulation of BSN cleavage by XerD and a concomitant deficiency in the resolution of chromosomal dimers at dif in vivo, although other XerD functions are not affected. |
| first_indexed | 2024-03-06T22:33:26Z |
| format | Journal article |
| id | oxford-uuid:59094477-7209-4987-9f06-eedf7552fddf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:33:26Z |
| publishDate | 2006 |
| publisher | Blackwell Publishing |
| record_format | dspace |
| spelling | oxford-uuid:59094477-7209-4987-9f06-eedf7552fddf2022-03-26T17:07:24ZDissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinaseJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:59094477-7209-4987-9f06-eedf7552fddfBiochemistryEnglishOxford University Research Archive - ValetBlackwell Publishing2006Yates, JZhekov, IBaker, REklund, BSherratt, DArciszewska, LSuccessful bacterial circular chromosome segregation requires that any dimeric chromosomes, which arise by crossing over during homologous recombination, are converted to monomers. Resolution of dimers to monomers requires the action of the XerCD site-specific recombinase at dif in the chromosome replication terminus region. This reaction requires the DNA translocase, FtsKc, which activates dimer resolution by catalysing an ATP hydrolysis-dependent switch in the catalytic state of the nucleoprotein recombination complex. We show that a 62-amino-acid fragment of FtsKc interacts directly with the XerD C-terminus in order to stimulate the cleavage by XerD of BSN, a dif-DNA suicide substrate containing a nick in the 'bottom' strand. The resulting recombinase-DNA covalent complex can undergo strand exchange with intact duplex dif in the absence of ATP. Ftskc-mediated stimulation of BSN cleavage by XerD requires synaptic complex formation. Mutational impairment of the XerD-FtsKc interaction leads to reduction in the in vitro stimulation of BSN cleavage by XerD and a concomitant deficiency in the resolution of chromosomal dimers at dif in vivo, although other XerD functions are not affected. |
| spellingShingle | Biochemistry Yates, J Zhekov, I Baker, R Eklund, B Sherratt, D Arciszewska, L Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase |
| title | Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase |
| title_full | Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase |
| title_fullStr | Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase |
| title_full_unstemmed | Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase |
| title_short | Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase |
| title_sort | dissection of a functional interaction between the dna translocase ftsk and the xerd recombinase |
| topic | Biochemistry |
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