Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line.
The neurodegenerative process in Parkinson's disease (PD) is accompanied by the presence of a neuroinflammatory response, which has been suggested as one of the principal components involved in PD progression. In this report we assessed the inflammatory potential of alpha-synuclein, a protein c...
| Hauptverfasser: | , , , , |
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| Format: | Journal article |
| Sprache: | English |
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2011
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| _version_ | 1826273789281828864 |
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| author | Alvarez-Erviti, L Couch, Y Richardson, J Cooper, J Wood, M |
| author_facet | Alvarez-Erviti, L Couch, Y Richardson, J Cooper, J Wood, M |
| author_sort | Alvarez-Erviti, L |
| collection | OXFORD |
| description | The neurodegenerative process in Parkinson's disease (PD) is accompanied by the presence of a neuroinflammatory response, which has been suggested as one of the principal components involved in PD progression. In this report we assessed the inflammatory potential of alpha-synuclein, a protein central to PD pathogenesis, released by neurons on the mouse microglia cell line BV-2. BV-2 cells were treated with conditioned medium isolated from normal SH-SY5Y cells and clones that over-express WT or mutant A53T alpha-synuclein. Conditioned medium isolated from over-expressing clones induced the transcription and release of pro-inflammatory cytokines. Treatment of SH-SY5Y alpha-synuclein over-expressing cells with MPP+, the active metabolite of the neurotoxin MPTP, increased the inflammatory response in BV-2 cells. In contrast, the direct exposure of BV-2 cells to MPP+ failed to induce an inflammatory response. These results support the hypothesis that WT and A53T alpha-synuclein has an important role in the initiation and maintenance of inflammation in PD, through the activation of a pro-inflammatory response in microglial cells. |
| first_indexed | 2024-03-06T22:33:33Z |
| format | Journal article |
| id | oxford-uuid:5914a6b8-4d03-419c-a202-a7cb863c39f4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:33:33Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:5914a6b8-4d03-419c-a202-a7cb863c39f42022-03-26T17:07:41ZAlpha-synuclein release by neurons activates the inflammatory response in a microglial cell line.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5914a6b8-4d03-419c-a202-a7cb863c39f4EnglishSymplectic Elements at Oxford2011Alvarez-Erviti, LCouch, YRichardson, JCooper, JWood, MThe neurodegenerative process in Parkinson's disease (PD) is accompanied by the presence of a neuroinflammatory response, which has been suggested as one of the principal components involved in PD progression. In this report we assessed the inflammatory potential of alpha-synuclein, a protein central to PD pathogenesis, released by neurons on the mouse microglia cell line BV-2. BV-2 cells were treated with conditioned medium isolated from normal SH-SY5Y cells and clones that over-express WT or mutant A53T alpha-synuclein. Conditioned medium isolated from over-expressing clones induced the transcription and release of pro-inflammatory cytokines. Treatment of SH-SY5Y alpha-synuclein over-expressing cells with MPP+, the active metabolite of the neurotoxin MPTP, increased the inflammatory response in BV-2 cells. In contrast, the direct exposure of BV-2 cells to MPP+ failed to induce an inflammatory response. These results support the hypothesis that WT and A53T alpha-synuclein has an important role in the initiation and maintenance of inflammation in PD, through the activation of a pro-inflammatory response in microglial cells. |
| spellingShingle | Alvarez-Erviti, L Couch, Y Richardson, J Cooper, J Wood, M Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line. |
| title | Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line. |
| title_full | Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line. |
| title_fullStr | Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line. |
| title_full_unstemmed | Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line. |
| title_short | Alpha-synuclein release by neurons activates the inflammatory response in a microglial cell line. |
| title_sort | alpha synuclein release by neurons activates the inflammatory response in a microglial cell line |
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