COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils
<p style="text-align:justify;"> <b>Background:</b> Eosinophilic airway inflammation is observed in 10-30% of COPD subjects. Whether increased eosinophils or impairment in their clearance by macrophages is associated with the severity and frequency of exacerbations is unk...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
BioMed Central
2014
|
| _version_ | 1826273819985182720 |
|---|---|
| author | Eltboli, O Bafadhel, M Hollins, F Wright, A Hargadon, B Kulkarni, N Brightling, C |
| author_facet | Eltboli, O Bafadhel, M Hollins, F Wright, A Hargadon, B Kulkarni, N Brightling, C |
| author_sort | Eltboli, O |
| collection | OXFORD |
| description | <p style="text-align:justify;"> <b>Background:</b> Eosinophilic airway inflammation is observed in 10-30% of COPD subjects. Whether increased eosinophils or impairment in their clearance by macrophages is associated with the severity and frequency of exacerbations is unknown.<br/><br/> <b>Methods:</b> We categorised 103 COPD subjects into 4 groups determined by the upper limit of normal for their cytoplasmic macrophage red hue (<6%), an indirect measure of macrophage efferocytosis of eosinophils, and area under the curve sputum eosinophil count (≥3%/year). Eosinophil efferocytosis by monocyte-derived macrophages was studied in 17 COPD subjects and 8 normal controls.<br/><br/> <b>Results:</b> There were no differences in baseline lung function, health status or exacerbation frequency between the groups: A-low red hue, high sputum eosinophils (n = 10), B-high red hue, high sputum eosinophils (n = 16), C-low red hue, low sputum eosinophils (n = 19) and D- high red hue, low sputum eosinophils (n = 58). Positive bacterial culture was lower in groups A (10%) and B (6%) compared to C (44%) and D (21%) (p = 0.01). The fall in FEV1 from stable to exacerbation was greatest in group A (ΔFEV1 [95 % CI] -0.41 L [-0.65 to -0.17]) versus group B (-0.16 L [-0.32 to -0.011]), C (-0.11 L [-0.23 to -0.002]) and D (-0.16 L [-0.22 to -0.10]; p = 0.02). Macrophage efferocytosis of eosinophils was impaired in COPD versus controls (86 [75 to 92]% versus 93 [88 to 96]%; p = 0.028); was most marked in group A (71 [70 to 84]%; p = 0.0295) and was inversely correlated with exacerbation frequency (r = -0.63; p = 0.006).<br/><br/> <b>Conclusions:</b> Macrophage efferocytosis of eosinophils is impaired in COPD and is related to the severity and frequency of COPD exacerbations. </p> |
| first_indexed | 2024-03-06T22:34:01Z |
| format | Journal article |
| id | oxford-uuid:593f5f40-136c-4f8b-b74a-481cc7506668 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:34:01Z |
| publishDate | 2014 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:593f5f40-136c-4f8b-b74a-481cc75066682022-03-26T17:08:40ZCOPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophilsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:593f5f40-136c-4f8b-b74a-481cc7506668EnglishSymplectic Elements at OxfordBioMed Central2014Eltboli, OBafadhel, MHollins, FWright, AHargadon, BKulkarni, NBrightling, C <p style="text-align:justify;"> <b>Background:</b> Eosinophilic airway inflammation is observed in 10-30% of COPD subjects. Whether increased eosinophils or impairment in their clearance by macrophages is associated with the severity and frequency of exacerbations is unknown.<br/><br/> <b>Methods:</b> We categorised 103 COPD subjects into 4 groups determined by the upper limit of normal for their cytoplasmic macrophage red hue (<6%), an indirect measure of macrophage efferocytosis of eosinophils, and area under the curve sputum eosinophil count (≥3%/year). Eosinophil efferocytosis by monocyte-derived macrophages was studied in 17 COPD subjects and 8 normal controls.<br/><br/> <b>Results:</b> There were no differences in baseline lung function, health status or exacerbation frequency between the groups: A-low red hue, high sputum eosinophils (n = 10), B-high red hue, high sputum eosinophils (n = 16), C-low red hue, low sputum eosinophils (n = 19) and D- high red hue, low sputum eosinophils (n = 58). Positive bacterial culture was lower in groups A (10%) and B (6%) compared to C (44%) and D (21%) (p = 0.01). The fall in FEV1 from stable to exacerbation was greatest in group A (ΔFEV1 [95 % CI] -0.41 L [-0.65 to -0.17]) versus group B (-0.16 L [-0.32 to -0.011]), C (-0.11 L [-0.23 to -0.002]) and D (-0.16 L [-0.22 to -0.10]; p = 0.02). Macrophage efferocytosis of eosinophils was impaired in COPD versus controls (86 [75 to 92]% versus 93 [88 to 96]%; p = 0.028); was most marked in group A (71 [70 to 84]%; p = 0.0295) and was inversely correlated with exacerbation frequency (r = -0.63; p = 0.006).<br/><br/> <b>Conclusions:</b> Macrophage efferocytosis of eosinophils is impaired in COPD and is related to the severity and frequency of COPD exacerbations. </p> |
| spellingShingle | Eltboli, O Bafadhel, M Hollins, F Wright, A Hargadon, B Kulkarni, N Brightling, C COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils |
| title | COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils |
| title_full | COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils |
| title_fullStr | COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils |
| title_full_unstemmed | COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils |
| title_short | COPD exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils |
| title_sort | copd exacerbation severity and frequency is associated with impaired macrophage efferocytosis of eosinophils |
| work_keys_str_mv | AT eltbolio copdexacerbationseverityandfrequencyisassociatedwithimpairedmacrophageefferocytosisofeosinophils AT bafadhelm copdexacerbationseverityandfrequencyisassociatedwithimpairedmacrophageefferocytosisofeosinophils AT hollinsf copdexacerbationseverityandfrequencyisassociatedwithimpairedmacrophageefferocytosisofeosinophils AT wrighta copdexacerbationseverityandfrequencyisassociatedwithimpairedmacrophageefferocytosisofeosinophils AT hargadonb copdexacerbationseverityandfrequencyisassociatedwithimpairedmacrophageefferocytosisofeosinophils AT kulkarnin copdexacerbationseverityandfrequencyisassociatedwithimpairedmacrophageefferocytosisofeosinophils AT brightlingc copdexacerbationseverityandfrequencyisassociatedwithimpairedmacrophageefferocytosisofeosinophils |