A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae
Members of the peptidoglycan recognition protein (PGRP) family play essential roles in different manifestations of immune responses in insects. PGRP-LC, one of seven members of this family in the malaria vector Anopheles gambiae produced several spliced variants. Here we show that PGRP-LC, and not o...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2007
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| _version_ | 1826273963010949120 |
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| author | Lin, H Zhang, L Luna, C Hoa, N Zheng, L |
| author_facet | Lin, H Zhang, L Luna, C Hoa, N Zheng, L |
| author_sort | Lin, H |
| collection | OXFORD |
| description | Members of the peptidoglycan recognition protein (PGRP) family play essential roles in different manifestations of immune responses in insects. PGRP-LC, one of seven members of this family in the malaria vector Anopheles gambiae produced several spliced variants. Here we show that PGRP-LC, and not other members of the PGRP family nor the six members of the Gram-negative binding protein families, is required for the expression of antimicrobial peptide genes (such as CEC1 and GAMI) under the control of the Imd-Rel2 pathway in an A. gambiae cell line, 4a3A. PGRP-LC produces many splice variants that can be classified into three sub-groups (LC1, LC2 and LC3), based on the carboxyl terminal sequences. RNA interference against one LC1 sub-group resulted in dramatic reduction of CEC1 and GAM1. Over-expression of LC1a and to a lesser extent LC3a (a member of the LC1 and LC3 sub-group, respectively) in the 4a3A cell line enhances the expression of CEC1 and GAM1. These results demonstrate that the LC1-subgroup splice variants are essential for the expression of CEC1 and GAM1 in A. gambiae cell line. © Institute of Zoology, Chinese Academy of Sciences. |
| first_indexed | 2024-03-06T22:36:13Z |
| format | Journal article |
| id | oxford-uuid:59fdb2c3-be41-4c8e-93b0-378a82b4658d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:36:13Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:59fdb2c3-be41-4c8e-93b0-378a82b4658d2022-03-26T17:12:57ZA splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiaeJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:59fdb2c3-be41-4c8e-93b0-378a82b4658dEnglishSymplectic Elements at Oxford2007Lin, HZhang, LLuna, CHoa, NZheng, LMembers of the peptidoglycan recognition protein (PGRP) family play essential roles in different manifestations of immune responses in insects. PGRP-LC, one of seven members of this family in the malaria vector Anopheles gambiae produced several spliced variants. Here we show that PGRP-LC, and not other members of the PGRP family nor the six members of the Gram-negative binding protein families, is required for the expression of antimicrobial peptide genes (such as CEC1 and GAMI) under the control of the Imd-Rel2 pathway in an A. gambiae cell line, 4a3A. PGRP-LC produces many splice variants that can be classified into three sub-groups (LC1, LC2 and LC3), based on the carboxyl terminal sequences. RNA interference against one LC1 sub-group resulted in dramatic reduction of CEC1 and GAM1. Over-expression of LC1a and to a lesser extent LC3a (a member of the LC1 and LC3 sub-group, respectively) in the 4a3A cell line enhances the expression of CEC1 and GAM1. These results demonstrate that the LC1-subgroup splice variants are essential for the expression of CEC1 and GAM1 in A. gambiae cell line. © Institute of Zoology, Chinese Academy of Sciences. |
| spellingShingle | Lin, H Zhang, L Luna, C Hoa, N Zheng, L A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae |
| title | A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae |
| title_full | A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae |
| title_fullStr | A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae |
| title_full_unstemmed | A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae |
| title_short | A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae |
| title_sort | splice variant of pgrp lc required for expression of antimicrobial peptides in anopheles gambiae |
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