Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone
Leishmania promastigote parasites have a flagellum, which protrudes from the flagellar pocket at the cell anterior, yet, surprisingly, have homologs of many flagellum attachment zone (FAZ) proteins – proteins used in the related Trypanosoma species to laterally attach the flagellum to the cell body...
| Main Authors: | , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Company of Biologists
2016
|
| _version_ | 1826311829427585024 |
|---|---|
| author | Wheeler, RJ Sunter, JD Gull, K |
| author_facet | Wheeler, RJ Sunter, JD Gull, K |
| author_sort | Wheeler, RJ |
| collection | OXFORD |
| description | Leishmania promastigote parasites have a flagellum, which protrudes from the flagellar pocket at the cell anterior, yet, surprisingly, have homologs of many flagellum attachment zone (FAZ) proteins – proteins used in the related Trypanosoma species to laterally attach the flagellum to the cell body from the flagellar pocket to the cell posterior. Here, we use seven Leishmania mexicana cell lines that expressed eYFP fusions of FAZ protein homologs to show that the Leishmania flagellar pocket includes a FAZ structure. Electron tomography revealed a precisely defined 3D organisation for both the flagellar pocket and FAZ, with striking similarities to those of Trypanosoma brucei. Expression of two T. brucei FAZ proteins in L. mexicana showed that T. brucei FAZ proteins can assemble into the Leishmania FAZ structure. Leishmania therefore have a previously unrecognised FAZ structure, which we show undergoes major structural reorganisation in the transition from the promastigote (sandfly vector) to amastigote (in mammalian macrophages). Morphogenesis of the Leishmania flagellar pocket, a structure important for pathogenicity, is therefore intimately associated with a FAZ; a finding with implications for understanding shape changes involving component modules during evolution. |
| first_indexed | 2024-03-07T08:17:05Z |
| format | Journal article |
| id | oxford-uuid:5a43a565-897d-4356-bbf9-cae7207c49d5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:17:05Z |
| publishDate | 2016 |
| publisher | Company of Biologists |
| record_format | dspace |
| spelling | oxford-uuid:5a43a565-897d-4356-bbf9-cae7207c49d52024-01-08T10:46:48ZFlagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zoneJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5a43a565-897d-4356-bbf9-cae7207c49d5EnglishSymplectic Elements at OxfordCompany of Biologists2016Wheeler, RJSunter, JDGull, KLeishmania promastigote parasites have a flagellum, which protrudes from the flagellar pocket at the cell anterior, yet, surprisingly, have homologs of many flagellum attachment zone (FAZ) proteins – proteins used in the related Trypanosoma species to laterally attach the flagellum to the cell body from the flagellar pocket to the cell posterior. Here, we use seven Leishmania mexicana cell lines that expressed eYFP fusions of FAZ protein homologs to show that the Leishmania flagellar pocket includes a FAZ structure. Electron tomography revealed a precisely defined 3D organisation for both the flagellar pocket and FAZ, with striking similarities to those of Trypanosoma brucei. Expression of two T. brucei FAZ proteins in L. mexicana showed that T. brucei FAZ proteins can assemble into the Leishmania FAZ structure. Leishmania therefore have a previously unrecognised FAZ structure, which we show undergoes major structural reorganisation in the transition from the promastigote (sandfly vector) to amastigote (in mammalian macrophages). Morphogenesis of the Leishmania flagellar pocket, a structure important for pathogenicity, is therefore intimately associated with a FAZ; a finding with implications for understanding shape changes involving component modules during evolution. |
| spellingShingle | Wheeler, RJ Sunter, JD Gull, K Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone |
| title | Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone |
| title_full | Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone |
| title_fullStr | Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone |
| title_full_unstemmed | Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone |
| title_short | Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone |
| title_sort | flagellar pocket restructuring through the leishmania life cycle involves a discrete flagellum attachment zone |
| work_keys_str_mv | AT wheelerrj flagellarpocketrestructuringthroughtheleishmanialifecycleinvolvesadiscreteflagellumattachmentzone AT sunterjd flagellarpocketrestructuringthroughtheleishmanialifecycleinvolvesadiscreteflagellumattachmentzone AT gullk flagellarpocketrestructuringthroughtheleishmanialifecycleinvolvesadiscreteflagellumattachmentzone |