The problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysis
Advances in NGS sequencing technologies, improved laboratory protocols and new bioinformatic workflows have seen huge increases in ancient DNA (aDNA) research on archaeological materials. A large proportion of aDNA work now utilises the petrous portion of the temporal bone (pars petrosa), which is r...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Taylor and Francis
2020
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| _version_ | 1797107478848077824 |
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| author | Charlton, S Booth, T Barnes, I |
| author_facet | Charlton, S Booth, T Barnes, I |
| author_sort | Charlton, S |
| collection | OXFORD |
| description | Advances in NGS sequencing technologies, improved laboratory protocols and new bioinformatic workflows have seen huge increases in ancient DNA (aDNA) research on archaeological materials. A large proportion of aDNA work now utilises the petrous portion of the temporal bone (pars petrosa), which is recognised as an excellent skeletal element for long-term ancient endogenous (host) DNA survival. This has been significant due to the often low endogenous content of other skeletal elements, meaning that large amounts of sequencing are frequently required to obtain sufficient genetic coverage. However, exclusive sampling of the petrous for aDNA analysis introduces a new set of potential biases into our scientific studies -- and these issues are yet to be considered by ancient DNA researchers. This paper aims to outline the possible biases of utilising petrous bones to undertake aDNA analyses, and highlight how these complications may potentially be overcome in future research. |
| first_indexed | 2024-03-07T07:15:10Z |
| format | Journal article |
| id | oxford-uuid:5a631361-7f9d-4763-ac0a-027387552ca9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:15:10Z |
| publishDate | 2020 |
| publisher | Taylor and Francis |
| record_format | dspace |
| spelling | oxford-uuid:5a631361-7f9d-4763-ac0a-027387552ca92022-08-10T08:30:46ZThe problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5a631361-7f9d-4763-ac0a-027387552ca9EnglishSymplectic Elements at OxfordTaylor and Francis2020Charlton, SBooth, TBarnes, IAdvances in NGS sequencing technologies, improved laboratory protocols and new bioinformatic workflows have seen huge increases in ancient DNA (aDNA) research on archaeological materials. A large proportion of aDNA work now utilises the petrous portion of the temporal bone (pars petrosa), which is recognised as an excellent skeletal element for long-term ancient endogenous (host) DNA survival. This has been significant due to the often low endogenous content of other skeletal elements, meaning that large amounts of sequencing are frequently required to obtain sufficient genetic coverage. However, exclusive sampling of the petrous for aDNA analysis introduces a new set of potential biases into our scientific studies -- and these issues are yet to be considered by ancient DNA researchers. This paper aims to outline the possible biases of utilising petrous bones to undertake aDNA analyses, and highlight how these complications may potentially be overcome in future research. |
| spellingShingle | Charlton, S Booth, T Barnes, I The problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysis |
| title | The problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysis |
| title_full | The problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysis |
| title_fullStr | The problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysis |
| title_full_unstemmed | The problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysis |
| title_short | The problem with petrous? a consideration of the potential biases in the utilisation of pars petrosa for ancient DNA analysis |
| title_sort | problem with petrous a consideration of the potential biases in the utilisation of pars petrosa for ancient dna analysis |
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