2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation.
The phosphoramidite monomer of the C-nucleoside 2'-aminoethoxy-2-amino-3-methylpyridine (AE-MAP) has been synthesized for the first time and incorporated into triplex-forming oligonucleotides (TFOs). Ultraviolet melting and DNase I footprinting studies show that AE-MAP is a potent triplex-stabi...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Published: |
2011
|
| _version_ | 1797070388222492672 |
|---|---|
| author | Lou, C Shelbourne, M Fox, K Brown, T |
| author_facet | Lou, C Shelbourne, M Fox, K Brown, T |
| author_sort | Lou, C |
| collection | OXFORD |
| description | The phosphoramidite monomer of the C-nucleoside 2'-aminoethoxy-2-amino-3-methylpyridine (AE-MAP) has been synthesized for the first time and incorporated into triplex-forming oligonucleotides (TFOs). Ultraviolet melting and DNase I footprinting studies show that AE-MAP is a potent triplex-stabilizing monomer that is selective for GC base pairs. TFOs containing AE-MAP bind with high affinity to duplexes but only weakly to single stranded DNA. In addition, AE-MAP confers high nuclease resistance on oligonucleotides. TFOs containing AE-MAP have potential for gene knock-out and gene expression studies. Copyright © 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| first_indexed | 2024-03-06T22:38:05Z |
| format | Journal article |
| id | oxford-uuid:5a9cd310-1090-4eca-850f-5f4227925c61 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T22:38:05Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:5a9cd310-1090-4eca-850f-5f4227925c612022-03-26T17:16:49Z2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5a9cd310-1090-4eca-850f-5f4227925c61Symplectic Elements at Oxford2011Lou, CShelbourne, MFox, KBrown, TThe phosphoramidite monomer of the C-nucleoside 2'-aminoethoxy-2-amino-3-methylpyridine (AE-MAP) has been synthesized for the first time and incorporated into triplex-forming oligonucleotides (TFOs). Ultraviolet melting and DNase I footprinting studies show that AE-MAP is a potent triplex-stabilizing monomer that is selective for GC base pairs. TFOs containing AE-MAP bind with high affinity to duplexes but only weakly to single stranded DNA. In addition, AE-MAP confers high nuclease resistance on oligonucleotides. TFOs containing AE-MAP have potential for gene knock-out and gene expression studies. Copyright © 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| spellingShingle | Lou, C Shelbourne, M Fox, K Brown, T 2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation. |
| title | 2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation. |
| title_full | 2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation. |
| title_fullStr | 2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation. |
| title_full_unstemmed | 2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation. |
| title_short | 2'-Aminoethoxy-2-amino-3-methylpyridine in triplex-forming oligonucleotides: high affinity, selectivity and resistance to enzymatic degradation. |
| title_sort | 2 aminoethoxy 2 amino 3 methylpyridine in triplex forming oligonucleotides high affinity selectivity and resistance to enzymatic degradation |
| work_keys_str_mv | AT louc 2aminoethoxy2amino3methylpyridineintriplexformingoligonucleotideshighaffinityselectivityandresistancetoenzymaticdegradation AT shelbournem 2aminoethoxy2amino3methylpyridineintriplexformingoligonucleotideshighaffinityselectivityandresistancetoenzymaticdegradation AT foxk 2aminoethoxy2amino3methylpyridineintriplexformingoligonucleotideshighaffinityselectivityandresistancetoenzymaticdegradation AT brownt 2aminoethoxy2amino3methylpyridineintriplexformingoligonucleotideshighaffinityselectivityandresistancetoenzymaticdegradation |