Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man.
We examined the timing of apoptosis and the expression of the DNA repair proteins poly(ADP-ribose) polymerase (PARP) and Ku80 in sections of frontal and temporal lobes from patients who had suffered severe brain ischaemia due to a cardiac arrent. In situ end-labelling (ISEL) was used to detect apopt...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1998
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| _version_ | 1797070438092767232 |
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| author | Love, S Barber, R Wilcock, G |
| author_facet | Love, S Barber, R Wilcock, G |
| author_sort | Love, S |
| collection | OXFORD |
| description | We examined the timing of apoptosis and the expression of the DNA repair proteins poly(ADP-ribose) polymerase (PARP) and Ku80 in sections of frontal and temporal lobes from patients who had suffered severe brain ischaemia due to a cardiac arrent. In situ end-labelling (ISEL) was used to detect apoptotic cells, and immunohistochemistry to assess PARP and Ku80. ISEL of scattered neurons and glia was demonstrable predominantly during the first 24 h after ischaemia. PARP and Ku80 immunoreactivity increased markedly after cerebral ischaemia, PARP particularly in the regions of greatest susceptibility to hypoxic injury: the CA1 field of the hippocampus and the depths of neocortical sulci. The up-regulation of PARP is in keeping with experimental observations concerning the key role of this enzyme in mediating ischaemic cell death. |
| first_indexed | 2024-03-06T22:38:49Z |
| format | Journal article |
| id | oxford-uuid:5ad63119-507a-40f8-b545-23d878d70d2e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:38:49Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:5ad63119-507a-40f8-b545-23d878d70d2e2022-03-26T17:18:18ZApoptosis and expression of DNA repair proteins in ischaemic brain injury in man.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5ad63119-507a-40f8-b545-23d878d70d2eEnglishSymplectic Elements at Oxford1998Love, SBarber, RWilcock, GWe examined the timing of apoptosis and the expression of the DNA repair proteins poly(ADP-ribose) polymerase (PARP) and Ku80 in sections of frontal and temporal lobes from patients who had suffered severe brain ischaemia due to a cardiac arrent. In situ end-labelling (ISEL) was used to detect apoptotic cells, and immunohistochemistry to assess PARP and Ku80. ISEL of scattered neurons and glia was demonstrable predominantly during the first 24 h after ischaemia. PARP and Ku80 immunoreactivity increased markedly after cerebral ischaemia, PARP particularly in the regions of greatest susceptibility to hypoxic injury: the CA1 field of the hippocampus and the depths of neocortical sulci. The up-regulation of PARP is in keeping with experimental observations concerning the key role of this enzyme in mediating ischaemic cell death. |
| spellingShingle | Love, S Barber, R Wilcock, G Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man. |
| title | Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man. |
| title_full | Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man. |
| title_fullStr | Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man. |
| title_full_unstemmed | Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man. |
| title_short | Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man. |
| title_sort | apoptosis and expression of dna repair proteins in ischaemic brain injury in man |
| work_keys_str_mv | AT loves apoptosisandexpressionofdnarepairproteinsinischaemicbraininjuryinman AT barberr apoptosisandexpressionofdnarepairproteinsinischaemicbraininjuryinman AT wilcockg apoptosisandexpressionofdnarepairproteinsinischaemicbraininjuryinman |