Medium-throughput production of recombinant human proteins: ligation-independent cloning.
Structural genomics groups have identified the need to generate multiple truncated versions of each target to improve their success in producing a well-expressed, soluble, and stable protein and one that crystallizes and diffracts to a sufficient resolution for structural determination. At the SGC,...
Main Authors: | Strain-Damerell, C, Mahajan, P, Gileadi, O, Burgess-Brown, N |
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Format: | Journal article |
Language: | English |
Published: |
2014
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