Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells.
Uptake of orthophosphate (Pi) by osteoblast-like cells is known to be stimulated by parathyroid hormone (PTH), but effects on intracellular [Pi] have not been investigated. Here we show in rat osteoblast-like cells (UMR 106-06) that PTH (10(-11) to 10(-7) M) increases both 32Pi uptake and cellular [...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1993
|
| _version_ | 1797070734514716672 |
|---|---|
| author | Ahmado, A Khouja, H Kemp, G Guilland-Cumming, D Russell, R Bevington, A |
| author_facet | Ahmado, A Khouja, H Kemp, G Guilland-Cumming, D Russell, R Bevington, A |
| author_sort | Ahmado, A |
| collection | OXFORD |
| description | Uptake of orthophosphate (Pi) by osteoblast-like cells is known to be stimulated by parathyroid hormone (PTH), but effects on intracellular [Pi] have not been investigated. Here we show in rat osteoblast-like cells (UMR 106-06) that PTH (10(-11) to 10(-7) M) increases both 32Pi uptake and cellular [Pi] by up to 50 per cent. 1,25 Dihydroxyvitamin D3 (1,25D) (10(-12) to 10(-6) M) and salmon calcitonin (CT) (10(-12) to 10(-6) g ml-1) also increased cellular [Pi] (by up to 60 per cent), but the percentage increases in total cellular 32Pi uptake were smaller. The effects of 1,25D were transient (observable at 80 min and 6 h but not 24 h), and were also observed with 24,25 dihydroxy- and 25 hydroxyvitamin D3. Transient degradation of organic phosphorus pools to Pi might contribute to this increased [Pi]. These pools remain to be identified but were not shown to be phospholipids. Foetal bovine serum also affected cellular [Pi]. Care is therefore needed in distinguishing direct hormonal effects on cellular [Pi] from indirect effects arising from changes in the rate of cell growth. |
| first_indexed | 2024-03-06T22:43:10Z |
| format | Journal article |
| id | oxford-uuid:5c4979fb-ae09-43c1-bc83-ac68eb748cc0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:43:10Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | oxford-uuid:5c4979fb-ae09-43c1-bc83-ac68eb748cc02022-03-26T17:27:12ZCalciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5c4979fb-ae09-43c1-bc83-ac68eb748cc0EnglishSymplectic Elements at Oxford1993Ahmado, AKhouja, HKemp, GGuilland-Cumming, DRussell, RBevington, AUptake of orthophosphate (Pi) by osteoblast-like cells is known to be stimulated by parathyroid hormone (PTH), but effects on intracellular [Pi] have not been investigated. Here we show in rat osteoblast-like cells (UMR 106-06) that PTH (10(-11) to 10(-7) M) increases both 32Pi uptake and cellular [Pi] by up to 50 per cent. 1,25 Dihydroxyvitamin D3 (1,25D) (10(-12) to 10(-6) M) and salmon calcitonin (CT) (10(-12) to 10(-6) g ml-1) also increased cellular [Pi] (by up to 60 per cent), but the percentage increases in total cellular 32Pi uptake were smaller. The effects of 1,25D were transient (observable at 80 min and 6 h but not 24 h), and were also observed with 24,25 dihydroxy- and 25 hydroxyvitamin D3. Transient degradation of organic phosphorus pools to Pi might contribute to this increased [Pi]. These pools remain to be identified but were not shown to be phospholipids. Foetal bovine serum also affected cellular [Pi]. Care is therefore needed in distinguishing direct hormonal effects on cellular [Pi] from indirect effects arising from changes in the rate of cell growth. |
| spellingShingle | Ahmado, A Khouja, H Kemp, G Guilland-Cumming, D Russell, R Bevington, A Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells. |
| title | Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells. |
| title_full | Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells. |
| title_fullStr | Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells. |
| title_full_unstemmed | Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells. |
| title_short | Calciotropic hormones raise the chemically detectable [Pi] in UMR 106-06 osteoblast-like cells. |
| title_sort | calciotropic hormones raise the chemically detectable pi in umr 106 06 osteoblast like cells |
| work_keys_str_mv | AT ahmadoa calciotropichormonesraisethechemicallydetectablepiinumr10606osteoblastlikecells AT khoujah calciotropichormonesraisethechemicallydetectablepiinumr10606osteoblastlikecells AT kempg calciotropichormonesraisethechemicallydetectablepiinumr10606osteoblastlikecells AT guillandcummingd calciotropichormonesraisethechemicallydetectablepiinumr10606osteoblastlikecells AT russellr calciotropichormonesraisethechemicallydetectablepiinumr10606osteoblastlikecells AT bevingtona calciotropichormonesraisethechemicallydetectablepiinumr10606osteoblastlikecells |