Fluorescence-based active site probes for profiling deubiquitinating enzymes.
Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating...
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Bibliographic Details
| Main Authors: |
McGouran, J,
Kramer, H,
Mackeen, M,
di Gleria, K,
Altun, M,
Kessler, B |
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| Format: | Journal article
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| Language: | English |
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| Published: |
2012
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