Fluorescence-based active site probes for profiling deubiquitinating enzymes.
Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2012
|
| _version_ | 1797070760095776768 |
|---|---|
| author | McGouran, J Kramer, H Mackeen, M di Gleria, K Altun, M Kessler, B |
| author_facet | McGouran, J Kramer, H Mackeen, M di Gleria, K Altun, M Kessler, B |
| author_sort | McGouran, J |
| collection | OXFORD |
| description | Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging. |
| first_indexed | 2024-03-06T22:43:33Z |
| format | Journal article |
| id | oxford-uuid:5c653a22-c91e-4b24-9baf-a700e4729434 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:43:33Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:5c653a22-c91e-4b24-9baf-a700e47294342022-03-26T17:27:57ZFluorescence-based active site probes for profiling deubiquitinating enzymes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5c653a22-c91e-4b24-9baf-a700e4729434EnglishSymplectic Elements at Oxford2012McGouran, JKramer, HMackeen, Mdi Gleria, KAltun, MKessler, BNovel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging. |
| spellingShingle | McGouran, J Kramer, H Mackeen, M di Gleria, K Altun, M Kessler, B Fluorescence-based active site probes for profiling deubiquitinating enzymes. |
| title | Fluorescence-based active site probes for profiling deubiquitinating enzymes. |
| title_full | Fluorescence-based active site probes for profiling deubiquitinating enzymes. |
| title_fullStr | Fluorescence-based active site probes for profiling deubiquitinating enzymes. |
| title_full_unstemmed | Fluorescence-based active site probes for profiling deubiquitinating enzymes. |
| title_short | Fluorescence-based active site probes for profiling deubiquitinating enzymes. |
| title_sort | fluorescence based active site probes for profiling deubiquitinating enzymes |
| work_keys_str_mv | AT mcgouranj fluorescencebasedactivesiteprobesforprofilingdeubiquitinatingenzymes AT kramerh fluorescencebasedactivesiteprobesforprofilingdeubiquitinatingenzymes AT mackeenm fluorescencebasedactivesiteprobesforprofilingdeubiquitinatingenzymes AT digleriak fluorescencebasedactivesiteprobesforprofilingdeubiquitinatingenzymes AT altunm fluorescencebasedactivesiteprobesforprofilingdeubiquitinatingenzymes AT kesslerb fluorescencebasedactivesiteprobesforprofilingdeubiquitinatingenzymes |