A study of surface receptors on rat T lymphocytes
<p>A double immunolabelling technique was developed to study microscopically the interactions between CD4, CD45 and the T cell receptor on the surface of rat T cells induced by the phenomenon of co-capping.</p> <p>It was found that both CD4 and CD45 passively co-cap with the activ...
| Główni autorzy: | , , , |
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| Format: | Praca dyplomowa |
| Język: | English |
| Wydane: |
1991
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| Hasła przedmiotowe: |
| _version_ | 1826274457775243264 |
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| author | Crocker, G Newton, M Martin, B Crocker, Glenn |
| author2 | Newton, M |
| author_facet | Newton, M Crocker, G Newton, M Martin, B Crocker, Glenn |
| author_sort | Crocker, G |
| collection | OXFORD |
| description | <p>A double immunolabelling technique was developed to study microscopically the interactions between CD4, CD45 and the T cell receptor on the surface of rat T cells induced by the phenomenon of co-capping.</p> <p>It was found that both CD4 and CD45 passively co-cap with the actively capped T cell receptor, that the T cell receptor and CD45 passively co-cap with CD4, but that neither CD4 nor the T cell receptor co-cap with CD45. Co-crosslinking and active capping of CD45 with either the T cell receptor or CD4 prevented CD4 or the T cell receptor respectively, from passively co-capping.</p> <p>These experiments were extended to study the effects of particular antibody crosslinking conditions on T cell proliferation and tyrosine phosphorylation. A correlation was found to exist between receptor distribution and the effects of particular antibody combinations on proliferation and tyrosine phosphorylation. The significance of this with respect to T cell activation is discussed.</p> <p>Finally, an observation is reported concerning the failure of some cell lines to cap antibody-crosslinked surface molecules. Preliminary investgations into the nature and extent of the phenomenon are described.</p> |
| first_indexed | 2024-03-06T22:43:43Z |
| format | Thesis |
| id | oxford-uuid:5c74a70b-1f5e-4c78-904f-7c4ff1b543a8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:43:43Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:5c74a70b-1f5e-4c78-904f-7c4ff1b543a82022-03-26T17:28:18ZA study of surface receptors on rat T lymphocytesThesishttp://purl.org/coar/resource_type/c_db06uuid:5c74a70b-1f5e-4c78-904f-7c4ff1b543a8ReceptorsT cellsEnglishPolonsky Theses Digitisation Project1991Crocker, GNewton, MMartin, BCrocker, GlennNewton, MMartin, B<p>A double immunolabelling technique was developed to study microscopically the interactions between CD4, CD45 and the T cell receptor on the surface of rat T cells induced by the phenomenon of co-capping.</p> <p>It was found that both CD4 and CD45 passively co-cap with the actively capped T cell receptor, that the T cell receptor and CD45 passively co-cap with CD4, but that neither CD4 nor the T cell receptor co-cap with CD45. Co-crosslinking and active capping of CD45 with either the T cell receptor or CD4 prevented CD4 or the T cell receptor respectively, from passively co-capping.</p> <p>These experiments were extended to study the effects of particular antibody crosslinking conditions on T cell proliferation and tyrosine phosphorylation. A correlation was found to exist between receptor distribution and the effects of particular antibody combinations on proliferation and tyrosine phosphorylation. The significance of this with respect to T cell activation is discussed.</p> <p>Finally, an observation is reported concerning the failure of some cell lines to cap antibody-crosslinked surface molecules. Preliminary investgations into the nature and extent of the phenomenon are described.</p> |
| spellingShingle | Receptors T cells Crocker, G Newton, M Martin, B Crocker, Glenn A study of surface receptors on rat T lymphocytes |
| title | A study of surface receptors on rat T lymphocytes |
| title_full | A study of surface receptors on rat T lymphocytes |
| title_fullStr | A study of surface receptors on rat T lymphocytes |
| title_full_unstemmed | A study of surface receptors on rat T lymphocytes |
| title_short | A study of surface receptors on rat T lymphocytes |
| title_sort | study of surface receptors on rat t lymphocytes |
| topic | Receptors T cells |
| work_keys_str_mv | AT crockerg astudyofsurfacereceptorsonrattlymphocytes AT newtonm astudyofsurfacereceptorsonrattlymphocytes AT martinb astudyofsurfacereceptorsonrattlymphocytes AT crockerglenn astudyofsurfacereceptorsonrattlymphocytes AT crockerg studyofsurfacereceptorsonrattlymphocytes AT newtonm studyofsurfacereceptorsonrattlymphocytes AT martinb studyofsurfacereceptorsonrattlymphocytes AT crockerglenn studyofsurfacereceptorsonrattlymphocytes |