Long-term human T-helper lines producing specific helper factor reactive to influenza virus.
Our understanding of the mechanism of T-cell help in antibody production in the mouse has been improved by the production of long-term lines of T cells, either using T-cell growth factor (TCGF or IL-2) or by creating T-cell hybridomas. Such cells have been shown to produce non-antigen-specific 1.2 o...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1981
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| _version_ | 1797070789229412352 |
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| author | Fischer, A Beverley, P Feldmann, M |
| author_facet | Fischer, A Beverley, P Feldmann, M |
| author_sort | Fischer, A |
| collection | OXFORD |
| description | Our understanding of the mechanism of T-cell help in antibody production in the mouse has been improved by the production of long-term lines of T cells, either using T-cell growth factor (TCGF or IL-2) or by creating T-cell hybridomas. Such cells have been shown to produce non-antigen-specific 1.2 or antigen-specific helper factors with genetically restricted or unrestricted activity. However, the factors are still not well characterized. We have examined the role of human T-helper cells and their soluble factors in inducing antibody production, initially in a xenogeneic system, using mouse B cells. Using a human in vitro secondary antibody response system for influenza virus we have investigated the effect of helper factors on human B cells. We report here the production of long-term cultures of antigen-specific human helper cells. Both the cells and the high-potency antigen-specific helper factor (HF) which they produce act on human B cells in a genetically restricted manner. Preliminary mapping of the restriction indicates associations, but not identity, with HLA-DR. |
| first_indexed | 2024-03-06T22:43:59Z |
| format | Journal article |
| id | oxford-uuid:5c886cbd-fdfb-4367-8ebb-76e4740c3e83 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:43:59Z |
| publishDate | 1981 |
| record_format | dspace |
| spelling | oxford-uuid:5c886cbd-fdfb-4367-8ebb-76e4740c3e832022-03-26T17:28:45ZLong-term human T-helper lines producing specific helper factor reactive to influenza virus.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5c886cbd-fdfb-4367-8ebb-76e4740c3e83EnglishSymplectic Elements at Oxford1981Fischer, ABeverley, PFeldmann, MOur understanding of the mechanism of T-cell help in antibody production in the mouse has been improved by the production of long-term lines of T cells, either using T-cell growth factor (TCGF or IL-2) or by creating T-cell hybridomas. Such cells have been shown to produce non-antigen-specific 1.2 or antigen-specific helper factors with genetically restricted or unrestricted activity. However, the factors are still not well characterized. We have examined the role of human T-helper cells and their soluble factors in inducing antibody production, initially in a xenogeneic system, using mouse B cells. Using a human in vitro secondary antibody response system for influenza virus we have investigated the effect of helper factors on human B cells. We report here the production of long-term cultures of antigen-specific human helper cells. Both the cells and the high-potency antigen-specific helper factor (HF) which they produce act on human B cells in a genetically restricted manner. Preliminary mapping of the restriction indicates associations, but not identity, with HLA-DR. |
| spellingShingle | Fischer, A Beverley, P Feldmann, M Long-term human T-helper lines producing specific helper factor reactive to influenza virus. |
| title | Long-term human T-helper lines producing specific helper factor reactive to influenza virus. |
| title_full | Long-term human T-helper lines producing specific helper factor reactive to influenza virus. |
| title_fullStr | Long-term human T-helper lines producing specific helper factor reactive to influenza virus. |
| title_full_unstemmed | Long-term human T-helper lines producing specific helper factor reactive to influenza virus. |
| title_short | Long-term human T-helper lines producing specific helper factor reactive to influenza virus. |
| title_sort | long term human t helper lines producing specific helper factor reactive to influenza virus |
| work_keys_str_mv | AT fischera longtermhumanthelperlinesproducingspecifichelperfactorreactivetoinfluenzavirus AT beverleyp longtermhumanthelperlinesproducingspecifichelperfactorreactivetoinfluenzavirus AT feldmannm longtermhumanthelperlinesproducingspecifichelperfactorreactivetoinfluenzavirus |