Different types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells.
Allospecific immune responses against the MHC of another individual are remarkably strong, due t a high number of responding T cell clones. Although it has been demonstrated that some allospecific cytotoxic T lymphocytes (CTL) recognize peptides presented by allogeneic MHC class I molecules, it has...
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Sprache: | English |
| Veröffentlicht: |
1991
|
| _version_ | 1826274580673593344 |
|---|---|
| author | Aosai, F Ohlen, C Ljunggren, H Höglund, P Franksson, L Ploegh, H Townsend, A Kärre, K Stauss, H |
| author_facet | Aosai, F Ohlen, C Ljunggren, H Höglund, P Franksson, L Ploegh, H Townsend, A Kärre, K Stauss, H |
| author_sort | Aosai, F |
| collection | OXFORD |
| description | Allospecific immune responses against the MHC of another individual are remarkably strong, due t a high number of responding T cell clones. Although it has been demonstrated that some allospecific cytotoxic T lymphocytes (CTL) recognize peptides presented by allogeneic MHC class I molecules, it has remained unclear whether MHC molecules can be recognized directly. We used the H-2b-derived murine lymphoma mutant RMA-S, which has a defect affecting peptide loading of class I molecules, to test whether recognition by allospecific CTL always requires the presence of peptides. Three types of anti-H-2Kb CTL clones can be distinguished by their ability to lyse RMA-S target cells. Type A CTL clones efficiently lyse these target cells, the lysis by type B CTL clones is inefficient, and type C clones fail to lyse RMA-S. Up-regulation of the levels of H-2Kb density improved lysis by type B clones, but did not lead to lysis by type C clones. Some type A and B CTL clones apparently can recognize class I molecules devoid of peptides, while others are likely to recognize peptides which are not affected by the presentation defect of RMA-S. We suggest that type C clones are specific for peptides which are not presented by the mutant cells. The results show that the majority of alloreactive CTL recognize peptide/MHC complexes, while some CTL behave as if they can recognize class I molecules in the absence of MHC-bound peptides. |
| first_indexed | 2024-03-06T22:45:36Z |
| format | Journal article |
| id | oxford-uuid:5d0ebee7-a20b-49fa-ab80-87ec0cd5a8f7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:45:36Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:5d0ebee7-a20b-49fa-ab80-87ec0cd5a8f72022-03-26T17:32:02ZDifferent types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:5d0ebee7-a20b-49fa-ab80-87ec0cd5a8f7EnglishSymplectic Elements at Oxford1991Aosai, FOhlen, CLjunggren, HHöglund, PFranksson, LPloegh, HTownsend, AKärre, KStauss, HAllospecific immune responses against the MHC of another individual are remarkably strong, due t a high number of responding T cell clones. Although it has been demonstrated that some allospecific cytotoxic T lymphocytes (CTL) recognize peptides presented by allogeneic MHC class I molecules, it has remained unclear whether MHC molecules can be recognized directly. We used the H-2b-derived murine lymphoma mutant RMA-S, which has a defect affecting peptide loading of class I molecules, to test whether recognition by allospecific CTL always requires the presence of peptides. Three types of anti-H-2Kb CTL clones can be distinguished by their ability to lyse RMA-S target cells. Type A CTL clones efficiently lyse these target cells, the lysis by type B CTL clones is inefficient, and type C clones fail to lyse RMA-S. Up-regulation of the levels of H-2Kb density improved lysis by type B clones, but did not lead to lysis by type C clones. Some type A and B CTL clones apparently can recognize class I molecules devoid of peptides, while others are likely to recognize peptides which are not affected by the presentation defect of RMA-S. We suggest that type C clones are specific for peptides which are not presented by the mutant cells. The results show that the majority of alloreactive CTL recognize peptide/MHC complexes, while some CTL behave as if they can recognize class I molecules in the absence of MHC-bound peptides. |
| spellingShingle | Aosai, F Ohlen, C Ljunggren, H Höglund, P Franksson, L Ploegh, H Townsend, A Kärre, K Stauss, H Different types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells. |
| title | Different types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells. |
| title_full | Different types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells. |
| title_fullStr | Different types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells. |
| title_full_unstemmed | Different types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells. |
| title_short | Different types of allospecific CTL clones identified by their ability to recognize peptide loading-defective target cells. |
| title_sort | different types of allospecific ctl clones identified by their ability to recognize peptide loading defective target cells |
| work_keys_str_mv | AT aosaif differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT ohlenc differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT ljunggrenh differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT hoglundp differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT frankssonl differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT ploeghh differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT townsenda differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT karrek differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells AT staussh differenttypesofallospecificctlclonesidentifiedbytheirabilitytorecognizepeptideloadingdefectivetargetcells |